rev:    February 8, 2004

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DATA SHEET: Sheep anti-Aspartate Transaminase/Glutamic Oxaloacetic Transaminase (GOT) (Pig Heart)

Catalog#: RDI-ASPRTTRabS    $438.00/vial

Also available

HRP conjugated: cat#RDI-ASPRTTRabS-HRP    $469.00/20mg vial

Biotin conjugated #RDI-ASPRTTRbS-BT             $469.00/10mg vial

Size: 50 mg (lyophilized)

Antibody Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: Pig Heart

Applications: suitable for immunoblotting (western or dot blot) or ELISA

Reactivity: This polyclonal antibody reacts with aspartate transaminase (glutamic- oxaloacetic transaminase) isolated from pig heart showing bands at 40kda, 29kda and 17kda under denaturing and reducing conditions of SDS-PAGE. GOT transaminates L-aspartate and is an important serum indicator and the antibody may facilitate serum level determinations. Reactivity with the enzyme from other sources is possible but has not been tested.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher. ELISA, immunoblotting.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstitution.

Purity and Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by mmunoelectrophoresis resulted in a single precipitin arc against anti- sheep Serum as well as purified and partially purified pig heart GOT . Cross reactivity against other sources of GOT are unknown.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


phone 408-780-0908

      or (978) 371-6446 or 408-780-0908

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