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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DATA SHEET:Mouse anti-ANKYRIN Monoclonal Antibody
Catalog#: RDI-ankyrabmX Price:$561.75/50UG VIAL
Background: -Ankyrin consists of a single 215Kda peptide known to be involved in the attachment of spectrin (or fodrin) containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g. lymphocytes, platelets, fibroblasts and endothelial tissues). Ankyrin has been shown to underlie membrane proteins including CD44, the voltage-dependent sodium channel, NA+/K+ ATPase and the anion exchanger protein. -Functional diversity between members of the ankyrin family is generated by the expression of multiple genes as well as alternative splicing genes as well as alternative splicing of pre-mRna's. It is believed that the formation of a direct connection between ankyrin and functionally important trans membrane proteins/membrane skeleton may be one of the earliest events to occur during signal transduction and cell activation.
Package Size: 100ul IgG2a (0.5mg/ml) in 10mM SODIUM PHOSPHATE, 150mM NaCl,
0.01% NaN3, pH7.5
Isotype: IgG2a (purified from culture supernatant of ankyrin hybridoma cell
line)
Antigen: purified human erythroid cell ankyrin
Specificity: reacts specifically with intracellular ankyrin (215 kDa) in human,
mouse, rabbit, rat and bovine cells and tissues. (for
histochemistry, requires permeabilization steps as 0.1% saponin)
Use: -western blot (approx 1:1000) with 2-3 hr RT or overnight 4 DEG C with
chemiluminescence detection
-immunoprecipitation
-cytochemistry, 2% PFA fixation
-flow cytometry (with permeabilization)
-ELISA
ref: -Bennet, V. Physiological Reviewers, 70:1029- 1054
-Srinivasan, Y. et al, Nature, 333:177-180, 1988.
-Morrow, J.S. et al, J. Cell Bio. 108:455-465, 1989
-Bourguignon, L.Y.W. et al, J. Biol. Chem, 266:11761-11765, 1991
-Lokeshwar, V.B. et al, Int. Rev. Cytol., 87, 17983-17989, 1991
-Bourguignon, L.Y.W. et al, Int. Rev Cytol, 87:195-224, 1984.
Storage: Store at -20 DEG C or lower. AVOID FREQUENT FREEZE THAW
CYCLES. Do not freeze diluted antibody.
Precautions: For In vitro research Use Only. Not for use inor on humans or
animals or for diagnostics. It is the responsibility of the user to comply with
all local/state and Federal rules in the use of this product. We are not
responsible for any patent infringements that might result with the use of or
derivation of this product.
Mouse anti-Ankyrin G
cat# RDI-ANKYRGabmX $484.00/100ug
Presentation: 100ug in 1ml(0.1mg/ml) PBS + 0.2%
gelatin and 0.1% sodium azide.
Immunogen: a synthetic peptide derived from the human Ankyrin G spectrin
binding domain
Clone: 463
Isotype: mIgG
Reactivity: By Western blot, recognizes the 190kDa
isoform of Ankyrin G. This antibody has been shown to work in
immunofluorescence applications on frozen tissue. AnlyrinG is a good
immunohistochemical marker for axon inital segments, nodes of Ranvier, and
epithelial cell-cell contract sites.
Species Reactivity: human, mouse, rat and dog
Uses: -Western blot: 1-3ug/ml (2-3 hours RT or overnight 4 DEG C with
chemiluminescence detection) pos control mouse or rat brain extract
-immunofluorescence (frozen tissue) 2-5ug/ml (pos control unmyelinated axons) using FITC detection
-histochemistry (frozen sections)
Suggestion Trial Protocol (must optimize for each method)
Note: The antibody is sensitive to fixation conditions. The following conditions are recommended:1%PFA for perfusion and 4 hours post-fixation (after perfusion). For sciatic nerve, 2% paraformaldehyde is recommended for 2 hours at 4 DEG C.
-for immunofluorescence:
a) permeabilize for 15min at 4 DEG C in PBS containing 0.5% Triton X-100, 0.2% Tween 20 and 10mg/ml BSA.
b)
C)Apply antibody in blocking buffer (PBS with 0.2% Tween-20 and 10mg/ml BSA)
d)Wash with wash buffer (PBS with 0.2% Tween-20) 3-5 times at 4 DEG C
e) Apply secondary antibody (recommend using a biotin labelled secondary) dissolved in PBS with 0.2% Tween-20
f) Wash with wash buffer (PBS with 0.2% Tween-20) 3-5 times at 4 DEG C
g) Apply Streptavidin:FITC for 2 hours at 4 DEG C
h) Wash with wash buffer (PBS with 0.2% Tween-20) 3-5 times at 4 DEG C.
i) Mount slides.
ref: -J. Biol Chem. 270(5)2352-2359 (1995)
-J. Cell Sci 111:2197-2207 (1998)
Storage: 2-8 deg c (up to one month) or aliquot and store at -20 DEG C in non-frost free freezers. Avoid frequent freeze thaw cycles.
Background:
For Research Use Only
-SEE ALSO ABS To Ankyrin B
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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