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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers
a wide line of antibodies. Since no one antibody works best for all
applications (neutralization, blotting, ELISA, etc), we offer many different
types of antibodies to help solve this problem. Please inquire for other
applications or types of antibodies not listed below.
Anti-Amylin Peptide
Monoclonal anti-Amylin Peptide
cat# RDI-AMYLINPabm $525.00/vial
Specificity:* recognizes Human Islet amyloid peptide
Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added.
Clone: R10/99
Isotype: mIgG1k
Antigen: synthetic peptide sequence (CATQRLANFLV) coupled to tuberculin
Hybridoma: mouse myeloma (SP2/0)
USES: -frozen section (not evaluated)
-Paraffin sections
-typical dilutions:1:50-1:100, 60 minute incubation at 25 DEG C. ABC detection
-western blot:not evaluated
Stain: cytoplasmic
Pos Controls: pancreas
Reconstitution: 1 ml distilled water. Let set at least 30
minutes. Tighten cap and mix gently.
Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at
4 DEG C for immediate use. For long term use, recommend aliquoting and store at
-20 DEG C. Avoid frequent freeze thaw cycles.
Background:Amylin is a pancreatic islet peptide with a
role in the maintenance of glucose homostasis. It is predominantly found in the
beta cells of the pancreas and to a lesser extent in the gastrointestinal tract
and nervous system. Amylin appears to work with insulin to regulate
plasma/glucose concentrations in the bloodstream, suppressing secretion of
glucagon after eating and restraining the rate of gastric emptying. Patients
with diabetes mellitus have a deficiency in the secretion of glucagon that
parallels the deficiency of insulin secretion. Amylin, when administered to
diabetics, restore, in a dose dependent way, those functions previously
suppressed. Two fragments of amylin have ben identified in vivo. One fragment
contains residues 17 to 37 of human amylin and the other contains residues 24
to 37. Amylin (24-37) peptide fragment forms amyloid deposits, however, smaller
fragments eg residues 20 to 29, are also capable of generating amyloid. A
soluble factor from pancreatic carcinoma cells selectively stimulates amylin
secretion from islet cells, explaining the detection of excessive quantities of
amylin found in cases of pancreatic cancer. As this increase in amylin
concentration is an early feature of pancreatic cancer. This antibody may prove
of value in studies to determine the level of staining for amylin in normal and
diabetic pancreas and pancreatic carcinomas.
References:
-Kruger D F et al, Clinical implications of amylin and amylin deficiency. Diabetes Educ 25(3):389-397 (1999)
-Nilsson MR et al, Analysis of amylin cleavage products provides new insights into the amyloidogenic region of human amylin. J Mol Biol 294(5):1375-1385 (1999)
-Ding X et al, Pancreatic cancer cells selectively stimulate islet beat cells to secrete amylin.. Gastroenterology 114(1):130- 138 91998)
-Guldobono, F. Amylin and Gastrointestinal activity. Gen Pharmacol 31(2):173-177 (1998)
FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICSFOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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