rev:       June 24, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Catalog Number: RDI-ALS25401    $315.00/100ug    $1875.00/1mg

Presentation: 100ug in 100 ul (1mg/ml) affinity purified rabbit IgG in PBS containing 0.02% sodium azide.

Lot Number:  see sheet with shipment or vial label

Description: The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Host Species: Rabbit

Specificity: Reacts with the Tet repressor protein.

Applications: Western blot - Use at 1: 1:1000 dilution.

Detection limit in ELISA: 0.2 ng

Detection limit in Western blot: 0.8 ng.

However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

References:

1. Pook, E., et al., Eur. J. Biochem. 258 (1998) 915 - 922

2. Hillen, W. and Berens, C., Annu. Rev. Microbiol. 48 (1994) 345 - 369

3. Gatz, C., Biospektrum 1 (1995) 23 - 29

4. Mayford, M., et al., Science 274 (1996) 1678

For In Vitro research use only-not for use in or on humans or animal-not for use in diagnostics

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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