rev:    November 17, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-AGTRPabGX       $438.00/50ug (50ul)

       RDI-AGTRPabGX1     $750.00/100ug (100ul)

Presentation: affinity purifed goat IgG in phosphatebuffered   saline (PBS)

IgG: Goat IgG

Reactivity: Human

Immunogen: Recombinant human Agouti-Related Protein.

Format: Liquid 1 mg/ ml

Applications: Immunohistochemistry: 5-15ug/ml

                       Western Blot: 0.1 - 0.2 µg/mL Only tested for Western using recombinant

                        ELISA: 0.5-1 µg/mL

Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Storage: Antibodies are stable for six months from date of receipt when stored at -20 ° C to -70 ° C.The   antibody can be stored at 2 ° - 8 ° C for 1 month without detectable loss of activity.Upon   receipt, antibody should be aliquotted and stored frozen at -20 ° C to -70 ° C in a manual   defrost freezer. Avoid repeated freeze?thaw cycles.

Notes: For western blot analysis, the antibody can be used at 0.1 -secondary reagents to detect recombinant human Agouti-Related Protein. The detection limit for recombinant human Agouti-Related Protein is approximately 1 ng/lane under non-reducing condiitons For immunohistochemistry, the antibody will detect human Agouti-Related Protein in paraffin-embedded tissue sections. The working dilution after antigen retrieval is 5 - 15 µg/mL

For Research Use Only

cat# RDI-MAGTRPabGX      $438.00/50ug (50ul)

        RDI-MAGTRPabGX1   $750.00/100ug (100ul)

Presentation: affinity purifed goat IgG in phosphate buffered saline (PBS)

IgG: Goat IgG

Reactivity: Rat,mouse

Immunogen: Recombinant mouse Agouti-Related Protein.

Format: Liquid 1 mg/ ml

Applications: Immunohistochemistry: 5-15ug/ml

                      Immunocytochemistry: 15ug/ml

                      Western Blot: 0.1 - 0.2 µg/mL Only tested for Western using recombinant

                         ELISA: 0.5-1 µg/mL

Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.

Storage: Antibodies are stable for six months from date of receipt when stored at -20 ° C to -70 ° C.The   antibody can be stored at 2 ° - 8 ° C for 1 month without detectable loss of activity.Upon   receipt, antibody should be aliquotted and stored frozen at -20 ° C to -70 ° C in a manual   defrost freezer. Avoid repeated freeze thaw cycles.

Notes: Western Blot: Only tested for Western using recombinant mouse Agouti-Related Protein

Antiserum was used on fixed tissue. This antibody can be used with the appropriate secondary reagents to detect Agouti-Related Protein on tissues dissected from animals that were fixed by vascular perfusion with 4% paraformaldehyde/PBS (pH 7.4) and followed by perfusion with a 10% sucrose solution in 0.1 M phosphate buffer (pH 7.2).

For Research Use Only

 see anti-goat secondary antibodies

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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