rev:    November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

see monoclonal and polyclonals below

-see also nicotinic acetylcholine receptor antibodies

cat# RDI-ACHERA1abm      $438.00/vial 50ug

       RDI-ACHERA1abm-1   $750.00/vial 150ug

Background: Acetylcholine is an amine neurotransmitter at the neuromuscular   junction. It is released from the presynaptic membrane of a   cholinergic synapse into the synaptic cleft. It   diffuses across the cleft and binds acetylcholine receptors (AChR)   on the postsynaptic membrane. Receptor binding induces   postsynaptic membrane depolarization and the generation of an action potential that produces effects such as muscle contraction. The AChR is a 250kDa pentameric complex of four transmembrane subunits in a stoichiometry of a2bgd. In response to ligand binding, all subunits participate in the formation of an integral cation channel. However, the acetylcholine binding site is primarily within the a subunit. Myasthenia gravis (MG) is an autoimmune condition in which AchR levels are decreased. Autoantibodies bind and crosslink the AchRs leading to their internalization and degradation. This results in a decreased number of functional AChRs. Patients develop muscular weakness and some voluntary muscle fatigue. However, development of MG is also affected by genetic factors. One of the allelic forms of the AChRa gene appears to significantly contribute to MG susceptibility.

isotype: mIgG2a

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment corresponding to amino acids 332-457 from rat ACHR alpha

Use: Suitable for western blot on rat and mouse using approx 1ug/ml on total cell lysate of BC3H1 cells (with enhanced chemiluminescece detection, 2-3 hour RT or overnight 4 DEG C.) Main band =Mw 49 kDa

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

General REF: Schroder B et al. 1994 J. Biol Chem 269:10407

Garchon, H J et al. 1994 Proc Natl Acad Sci USA 91:4668

-copyright by owner

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Recommneded positive western blotting Control:for anti-AChERA monoclonal

"BC3H1"

cell lysate is provided as 500 micrograms in 50ul (1mg/ml) in SDS-PAGE buffer (62 mM Tris pH 6.8, 2% SDS, 1% B- mercaptoethanol, 0.003% bromophenol blue, 5% glycerol . Use approx 5- 10ul per lane. DO NOT Boil or dilute before use. The control is derived from a mouse tumor elicited by nitrosoethylurea.

cell lysates available on request

cat#RDI-BC3H1/CPL $250.00/500ug vial

see http://www.researchd.com/controls/lysates.htm

cat#  RDI-ACHERBabm        $438.00/vial 50ug

        RDI-ACHERBabm-1     $750.00/vial 150ug

Background: The AChR is a 250kDa pentameric complex of four honologous trans membrane subunits in a stoichiometry of alpha beta gamma delta. Agrin is a protein that mediates nerve-inducing AChR aggregation at developing neuromuscular junctions. It has been shown that agrin induces phosphorylation of the beta, gamma, and delta subunits of AChRs in cultured myotubes. The Beta subunit is tyrosine phosphorylated while the gamma and delta subunits appear to be serine/threonine phosphorylated. The agrin-induced phosphorylation generally precedes receptor aggregation and inhibition of agrin-induced AChR aggregation and is also known to inhibit tyrosine phosphorylation of the B subunit. Thus agrin-induced tyrosine phosphorylation of the AChRB subunit appears to play a critical role in AChR aggregation. In addition, phosphorylation of the  AChR by tyrosine kinases has been correlated with a modulation of   the rapid rate of receptor desensitizaion and may play a role in the nerve-induced clustering of the AChR at the synapse. Two Src family protein tyrosine kinases, fyn, and Fyk, appear to be involved in the regulation of synaptic transmission at the neuromuscular junction by phosphorylating the AChR. The data suggest that fyn and Fyk can phosphorylate the AChR. The data suggest that fyn and Fyk can phosphorylate the AChR delta subunit and subsequently bind to delta through their SH2 domains. The B subunit induced tyrosine phosphorylation may proceed through a different set of protein tyrosine kinases.

isotype: mIgG2b

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a protein fragment corresponding to amino acids 62-224 from rat ACHR alpha

Use: Suitable for western blot on human and rat and using approx 0.5ug/ml a on total cell lysate of HELA cells (with enhanced chemiluminescence detection, 2-53 hour RT or overnigth 4 DEg C.) Main band =Mw 55 kDa Also suitable for immunofluorescence

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

General REF: Swope, S.L. et al 1994J. Biol Chem 266:7481

                     Hoch, W et al. 1994 J. Cell Biol 126:1

                     Wallace, B.G. 1994 J. Cell Biol 125:661

-copyright by owner

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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