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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

cat# RDI-ACHEXabM   $500.00/vial

Presentation: 200ul of PBS-diluted ascites containing 0.05% sodium azide

Clone: HR2

Isotype: mIgG2b

Immunogen: Purified human cerebellar acetylcholinesterase.

Species  Reactivity: Bovine, Feline, Guinea Pig, Human, Rabbit

Use: detects acetylcholinesterase (AChE) from human, rabbit, guinea pig, bovine and cat tissues. This antibody does not detect butyrylcholinesterase (BChE),

This mab has been successfully used in immunohistochemistry, immunoprecipitation and ELISA procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of AChE in human brain samples yields staining of nerve fibers and terminals

Applications: Immunohistochemistry (Frozen) 1:50

                       Immunoprecipitation Assay dependent

Background: Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft. .

References: Brain Research, 539: 233-238, 1991.

                   Cellular and Molecular Neurobiology, 8(1): 85-93, 1988.

                    J. Neural. Transm., 34: 139-1450, 1991.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-ACHERTabM   $500.00/vial

Presentation: 100ul of PBS-diluted ascites containing 0.05% sodium azide

Clone: ZR3

Isotype: mIgG2b

Immunogen: Purified rat brain acetylcholinesterase..

Species Reactivity: Feline, Guinea Pig, Rabbit,Rat

Use: -detects acetylcholinesterase (AChE) from feline, rat, rabbit and guinea pig tissues.

This Mab has been successfully used in immunohistochemistry and immunoprecipitation procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of Ach in rat brain with this Mab results in staining of nerve fibers and terminals. Reacts with both the soluble and membrane-associated AchE

Applications: Immunohistochemistry (Frozen) 1:1000

                       Immunoprecipitation Assay dependent

Background: Cholinergic neurotransmission occurs in motor, autonomic and   central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft. .

References: J. Neural. Transm., S34: 139-145, 1991.

                    J. Neurochem., 46(1): 280-287, 1986.

                    Neurosci., 54(1): 201-223, 1993

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-ACHEabm          $438.00/vial 50ug (0.2ml)

       RDI-ACHEabm-1       $750.00/vial 150ug (0.6ml)

Background: AChE (acetylcholinesterase) is a type B carboxylesterase that rapidly hydrolyzes acetylcholine in neuromuscular junctions and brain cholinergic synapses. In vertebrate muscle, AChEs exist in two broad structural classes, globular and asymmetric. The catalytic subunits of both classes are encoded by a single gene. AChE is assembled into multimeric catalytic subunits consisting of dimers and tetramers. The asymmetric form appears to be anchored to basal lamina components through a disulfide bonded collagen like peptide.The AChE globular form subunits can also associate with the cell membrane through either a disulfide-linked small inserted peptide or through a covalently attached glycophospholipid anchor. Currently, there is no direct evidence that these glycophospholipid-anchored forms exist in the chicken. Three classes of membrane-bound AChEs have been identified;A12, AChE, G2 AChE and G4 AChE. In mammals, the G2 AChE class is found in erythrocytes and is localized in membranes by a glycoinositol phospholipid covalently linked to the C-terminal amino acid of the catalytic subunits. the G4 AChE class is found in the mammalian central nervous system. These tetramers consist of two subunits linked by a direct intersubunit disulfide bond while the other two are disulfide linked to the membrane-binding, non catalytic subunit.

isotype: mIgG1

package: 50ug or 150ug purified IgG1 at 0.25mg/ml in 50% glycerol, 20mM sodium phosphate pH 7.5, 150mM NaCl, 1.5mM NaN3, 1mg/ml BSA

antigen: a 23.3kDa fragment corresponding to amino acids   411-609 of the human AChE protein

USe: Suitable for western blot on human, dog, rat, and   mouse, using approx 1 to 0.04ug/ml ab on total   cell lysate. Major band at Mw 68 kDa. Positive   control human SKN cells.
      -immunoprecipitation under native or denatured conditions

      -immunofluorescence

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Main REF: Michel, R.N. et al. (1994) J. Cell Biol. 127:1061

                 Tsim, K.W.K. et al. (1992) J. Cell Biol. 118:1201

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available: (please inquire)

monoclonal anti-AchR alpha cat#RDI-ACHRAabm $438.00/50ug

monoclonal anti-AchR beta cat#RDI-ACHRBabm $438.00/50ug

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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