rev: 5/16/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DATA SHEET: Rabbit
anti-Acid Phosphatase (potato)
Catalog#: RDI-ACDPHOSabr $281.00/vial
Also available HRP conjugated: cat#RDI-ACDPHOS-HRP $379.50/20mg
Biotin conj: cat#RDI-ACDPHOS-BT $312.00/10mg
Size: 50 mg (lyophilized)
Antibody Concentration: approx 10mg/ml
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2
Stablizer: None
Preservative: 0.01% (w/v) Sodium Azide
Antigen: acid phosphatase (potato)
Applications: suitable for immunoblotting (western under non-reducing
conditions) or dot blot),ELISA, immunoprecipitation, conjugation and most
immunological methods requiring high titer and specificity.
Reactivity: Reacts with acid phosphatase from potato. Cross reactivity with
acid phosphatase from other sources is likely. Under non-reducing conditions a
96kDa protein is reactive by western blotting. Acid phosphatase is a
heterodimer of 44kDa units. Antibody will recognize EI, EII and EIII forms of
the enzyme.
Recommended Dilutions: Not determined. Optimal titers for applications should
be determined by the researcher.
Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0
ml of deionized water (or equivalent). Centrifuge product if not completely
clear after standing at room temperature. This product is stable for several
weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For
extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of
freezing and thawing. Expiration date is one (1)year from date of
reconstituion.
Purity and Specificity: This product is an IgG fraction antibody purified from
monospecific antiserum by a multi-step process which includes delipidation,
salt fractionation and ion exchange chromatography followed by extensive
dialysis against the buffer stated above. Cross reactivity against acid
phosphatases from other sources may occur but have not been specifically
determined.
Precautions: For In vitro research Use Only. Not for use in or on humans or
animals or for diagnostics.
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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