rev:    December 5, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Product:               8-Hydroxyguanosine Antiserum

Cat No:                RDI-8OHGUANabG         $315.00/0.25ml

 Host:                   Goat

Clone:                 Polyclonal

 Immunogen:          8-Hydroxyguanosine-conjugate

 Form:                  Whole serum

 Concentration:      N/A

 Supplied as:          0.25ml

 Titer:                   Reported working dilution using neat serum:*

                                             -ELISA using immunizing peptide: 1:250,000

                                     -IHC using formalin-fixed, paraffin-embedded sections: 1:200

                                      -IHC using paraformaldehyde-fixed, frozen sections: N/D

                                       -Western blots: N/A

Specificity:           8-Hydroxy-2’-deoxyguanosine and 8-hydroxyguanosine.

 

Preservative:        0.1% Sodium Azide        ~

 

Storage:               Short term: Refrigerate at 4º C

Long term:             Freeze at -20º C.

 * Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.

FOR RESEARCH AND MANUFACTURING USE ONLY

8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease.

 

8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA.  Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation.  Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications.  In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.  Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other neutraceuticals.

 A goat antiserum to 8-hydroxyguanosine is currently available.  This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides.  This antibody has also been shown to immunolabel neurons using formalinfixed, paraffinembedded tissue sections.  This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.

 This antiserum was produced using proprietary methodology whereby the hapein is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the haptin.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  A control immune serum (Cat no. RDI-GPA018E - $315.00/500 �l) from a mock immunized animal is available to be used in conjunction with this antibody.

 

 

Alzheimer disease brain showing immunohistochemcal staining of oxidized RNA in neurons. Tissue sections were formalin-fixed, parafffin-embedded with either (A)  no pretreatment, or (B) pretreated with 10 ug/ml proteinase K for 40 minutes at 37°C. and stained  with at 1:200 dilution primary antiserum.
   
 


Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides.  Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity.  2-Deoxyadenosine, 7-methylguanosine,guanosine monophosphate and guanosine show no reactivity.

For Research Use Only-Not For Use in Diagnostics

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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