rev: 5/16/2008
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ANTIBODIES
(anti-Human
and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of
antibodies. Since no one antibody works best for all applications
(neutralization, blotting, histochemistry, ELISA, etc), we offer many different
types of antibodies to help solve this problem. Please inquire for other
applications or types of antibodies not listed below. Product: 8-Hydroxyguanosine
Antiserum
ELISA using immunizing
peptide: 1:250,000
v
-IHC using formalin-fixed,
paraffin-embedded sections: 1:200
v
-IHC using paraformaldehyde-fixed,
frozen sections: N/D
v
-Western blots: N/A
Specificity: 8-Hydroxy-2’-deoxyguanosine and
8-hydroxyguanosine.
Preservative: 0.1% Sodium Azide ~
Storage: Short term: Refrigerate at
4º C
Long term:
Freeze at -20º C.
*
Various assay conditions require that the optimum working concentrations be
determined by serial dilutions of this product.
FOR RESEARCH AND MANUFACTURING
USE ONLY
8-Hydroxydeoxyguanosine
(8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl
radicals that are formed as byproducts and intermediates of aerobic metabolism
and during oxidative stress. There is increasing evidence to support the
involvement of free radical reactions in the damage of biomolecules that
eventually lead to several diseases in humans, such as atherosclerosis,
cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis,
inflammation, diabetes, aging, and neurodegenerative conditions, such as
Alzheimer’s disease.
8OHdG has become increasing popular as a
sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that
8OHdG can be excreted in the urine, and that a significant increase is caused
by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with
oxidative stress and damage to DNA, which leads to degenerative disease states,
the development of an antibody that can be used to study DNA damage has
numerous applications. In addition to
the direct study of DNA damage within cells, this antibody has applications in
the development of immunoassays that can monitor 8OHdG excretion in the urine
and serve as a biomarker of oxidative stress.
Industrial uses may extend to the dietary supplement manufacturers, who
could benefit from an immunoassay that could be used to test the effectiveness
of antioxidants and other neutraceuticals.
A goat antiserum to 8-hydroxyguanosine is
currently available. This antiserum has
been shown by ELISA to be completely cross-reactive with
8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring
nucleotides. This antibody has also been
shown to immunolabel neurons using formalinfixed, paraffinembedded
tissue sections. This antibody should be
a valuable tool for scientists working to understand the role of free radical
damage in a number of human disease states.
This antiserum was produced using proprietary
methodology whereby the hapein is attached to a carrier that elicits minimal
immunoreactivity so that the antiserum has a higher degree of specificity for
the haptin. Since there is no
overwhelming production of interfering antibodies to the carrier, this
antiserum can routinely be used without further purification. A control immune serum (Cat no. RDI-GPA018E,
new cat#: 20R-CG007 - $346.50/500 µl) from a mock immunized animal is available
to be used in conjunction with this antibody.
Alzheimer disease brain showing
immunohistochemcal staining of oxidized RNA in neurons. Tissue
sections were formalin-fixed, parafffin-embedded with either
no pretreatment, or (B) pretreated with 10 ug/ml proteinase K for
40 minutes at 37°C. and stained with at 1:200 dilution primary antiserum.
Competitive ELISA using
the 8-OHG antibody demonstrates a high specificity towards
8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is
observed with 8-mercaptoguanosine while 8-bromoguanosine shows
some cross-reactivity. 2-Deoxyadenosine,
7-methylguanosine,guanosine monophosphate and guanosine show no
reactivity.
For Research Use
Only-Not For Use in Diagnostics
RDI Division of Fitzgerald Industries Intl
34
Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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