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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


NUP153

see some other abs to nuclear antigen from pdf list   nuclear.pdf  


Mab to nup153    Cat#RDI-PRO651141   $375.00

Clone Determination                 nup7A8
Category                                    Mouse monoclonal
Immunoglobulin Subclass          IgG1
Purification/Form                      Hybridoma culture supernatant
Immunogen                                 Nuclear matrix protein fraction prepared from rat liver nuclei

Description / Specificity           The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kD), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase.

Antigen Recognized in              Reactive with many species, including human, rodents

Species (tested so far)              (mouse, rat), marsupials (kangaroo rat), amphibia (xenopus, pleurodeles)

Application                               Immunofluorescence microscopy on frozen sections (also after fixation with                                                                         paraformaldehyde)

                                                  Immunoblotting (Western)

                                                  Immunoprecipitation

Working Dilution                      1:5 - 1:10 for immunohistochemistry

                                                  1:30 - 1:50 for Western blot (ECL method)

Incubation time                          1 h at RT

Storage At                                  2-8°C

Volume                                      5 mL (contains 0.09% NaN3)

References

Cordes VC, Reidenbach S, Köhler A, Stuurmann N, van Driel R, Franke WW: Intranuclear filaments containing a nuclearpore complex protein. J Cell Biol 123, 1333-1344 (1993).

Cordes VC, Gajewski A, Stumpp S, Krohne G: Immunocytochemistry of annulate lamellae: potential cell biological markers for studies of cell differentiation and pathology. Differentiation 58, 307-312 (1995).

Cordes VC, Reidenbach S, Franke WW: Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior. Cell Tissue Res 284, 177-191 (1996).

Hase ME, Cordes VC: Direct interaction with nup153 mediates binding of tpr to the periphery of the nuclear pore complex. Mol Biol Cell 14, 1923-1940 (2003).

Cat. No. RDI-PRO651141

For In Vitro research Use only-not for use in or on humans or animals-not for use in diagnostics


additional abs to Nuclear Antigens     nuclear.pdf  


RDI Div of Fitzgerlad Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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