rev:   October 1, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

see also anti-C3A,  anti-C3B-Alpha,  anti-C3b-Beta  , anti-C5, and anti-C5B neoepitope

Mab to Complement C1q

CatRDI-PRO610136      $375.00

Clone Determination       242G3

Category                         Mouse monoclonal

Immunoglobulin Class    IgG1

Purification                    Protein A affinity chromatography

Antigen                           Purified human complement C1q

Specificity                       Detection of C1q in serum, in serum-C1 and within immune complexes; inhibits hemolytic activity of C1 by binding to C1q in the liquid phase (antibody concentration necessary to inhibit 50% of hemolytic activity in the fluid phase is 0.85 µg/ml). With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5 µg/ml(cf.ref.1).

Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders.

Polypeptide Reacting        C-chain of complement C1q (25 kD in SDS-PAGE)

Antigen Recognized in       Human

Species (tested so far)

Application         Suitable for Inhibition experiments (e.g. hemolytic activity)

                             Immunoblotting (Western)

                              ELISA

Stability/Storage At 2-8°C one year after reconstitution

Working Dilution When reconstituted with 1ml distilled water, dilute further e.g. 1:100 for Western blotting (ECL method).

Incubation Time 1h at RT

Quantity 50 µg (lyoph.)

References

1. Heinz HP, Burger R, Golan DG, Loos M: Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q. J Immunology 132, 804-808 (1984).

2. Antes U, Heinz HP, Loos M: Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies. J Immunol Methods 74, 299-306 (1984).

3. Antes U, Heinz HP, Loos M: Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system. J Immunol Methods 102, 149-156 (1987).

For In Vitro Research Use Only

see also other complement antibodies from our alphabetical antibody list

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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