rev:      November 29, 2004

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                                            ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

see also   anti-LYVE-1,  anti-Podoplanin and  PROX-1 (Lymphatic endothelial cell markers)

sample facs experiments with podoplanin and slyve  facs-lec.pdf

"isolation of lymph endothelial cells (LEC) from dermal skin capillary ECs"

Cat#RDI-101M40X     $565.00/100ug

Description: Monoclonals were produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (podoplanin) protein as the immunizing antigen. The mouse antibody (18H5) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Host species        Mouse

Antigen:               gp36 (podoplanin)-expressing MDCK cells

Purification:         Protein G chromatography

Stabilizer:             none

Buffer:                  PBS pH 7.4 w/o preservative

Formulation:        lyophilized

Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for more than a year when kept at -20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C in the presence of a preservative. Recommend adding 0.1-1% BSA or other carrier to dilutions and addition of 0.05% sodium azide or other suitable preservative (if suitable for your use) Reconstituted antibody can also be aliquotted and stored at - 20°C to -70°C for at least 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Applications

Western Analysis: Use at a concentration of 1-2 µg/ml with the appropriate secondary reagents.

Immuofluorescence: Use at a concentration of 1-2 µg with the appropriate secondary reagents.

FACS analysis and cell sorting: Use at 3-20 µg/ml together with the appropriate secondary reagents

Immunohistochemistry: Use at 6-30 µg/ml.

-Optimal dilutions should be determined by each laboratory for each application.

Usage: Anti-human Podoplanin is offered for research use. Not for drug use. Not for human use.

Literature: [Breiteneder-Geleff et al., Am. J. Pathol. 151:1141,1997; Zimmer et al., Biochem. J. 341:277, 1999; Schacht et al., EMBO J. 22:3546-56, 2003]

** please note : always centrifuge vials before opening **

Cat#RDI-102PA40         $345.00/100ug

Cat#RDI-102PA40X       $562.00/200ug

Description: Produced from sera of rabbits immu nised with the recombinant ectodomain of Podoplanin (gp36). Podoplanin is a highhly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin colocalized with VEGFR-3/FLT-4, another marker for lymphatic endothelial cells.

Host species: Rabbit

Antigen: Recombinant ectodomain of human gp36 (podoplanin)

Purification: Protein A chromatography

Stabilizer: none

Buffer: lyophilized from PBS, pH 7.4 w/o preservative

Formulation: lyophilized rabbit IgG

Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for more than a year when kept at -20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquotted and stored at - 20°C to -70°C for at least 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Applications

Immunoprecipitation: For IP, the antibody should be used at a concentration of 2-4 µg/sample.

Western Analysis: For Western blot analysis, the antibody should be used at a concentration of 1-2 µg/ml with the appropriate secondary reagents.

Immuofluorescence: For immunofluorescence, the antibody should be used at a concentration of 1-2 µg.

Optimal dilutions should be determined by each laboratory for each application.

Literature:

[Breiteneder-Geleff et al., Am. J. Pathol. 151:1141,1997; Zimmer et al., Biochem. J. 341:277, 1999;

Schacht et al., EMBO J. 22:3546-56, 2003]

** please note : always centrifuge vials before opening **

For More literature refercnes see below

http://www.researchd.com/miscabs/102pa40.pdf

Cat#RDI-103M40X    $440.00/vial  100ug     

Description: Monoclonals were produced by immunizing Syrian hamsters with a murine thymic stromal cell line. This antibody recognizes mouse podoplanin. Hamster IgG antibody from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Host species      Syrian Hamster

Purification: Protein G chromatography

Stabilizer: none

Buffer: PBS pH 7.4 w/o preservative

Formulation: lyophilized

Reconstitution: When reconstituted in sterile water to a concentration of 1.0 mg/ml the antibody is stable for at least six weeks at 2-4°C.

Stability: The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0°C. Reconstituted antibody is stable at 4°C for >one month or can be stored in working aliquots at -20°C for more than six months.

Cross-Reactivity: The antibody does not cross-react with human podoplanin. Other species not tested.

Specificity: The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. It works well with paraffin sections. The antibody will detect a 36-40 kDa band in Western blotting. It also works with immunoprecipitation.

Immunostaining/Immunohistochemistry: Use diluted 1-10 µg/ml IgG

Western blotting: Use 1-10 µg/ml IgG

Immunoprecipitation: For IP use 1-5 µg IgG per 1 ml lysate or reaction volume.

Usage: For Research Use Only-not for use in or on humans or animals

Literature: [Schacht et al., EMBO J 22:3546, 2003]

See anti-syrian secondary abs at: http://www.researchd.com/rdiabs/2ndabs.htm

Goat anti-Syrian Hamster IgG (H+L):TRITC labeled  cat#RDI-107025142 $219.00/1ml affinity purified,**absorbed against bovine, human ,mouse, rat and rabbit serum proteins.

Goat anti-Syrian Hamster IgG (H+L):Biotin labeled  cat#RDI-107065142 $219.00/1ml affinity purified,**absorbed against bovine, human ,mouse, rat and rabbit serum proteins.

Goat anti-Syrian Hamster IgG (H+L):FITC labeled  cat#RDI-107095142 $219.00/1ml affinity purified,**absorbed against bovine, human ,mouse, rat and rabbit serum proteins.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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