rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see other MHC1 and other antibodies from main MHC list
Monoclonal anti-human MHC Class I (HLA-A, B and C)
cat# RDI-MHC1abm-3, new cat#: 10R-M109a $346.50/100ug
Presentation: 100ug in 0.1ml (1mg/ml)50 mM Sodium Phosphate pH 7.5, 100 mM
Potassium Chloride, 150mM NaCl, and 0.5mg/ml Gentamicin Sulfate.
Clone: 3F10
Isotype: Mouse IgG2a
Immunogen: Human HSB-2 T cell line
Reactivity: Antibody 3F10 recognizes a monomorphic Class I epitope on the a
chain. Antibody 3F10 (whole molecule) is cytotoxic in the presence of
complement (1).
STORAGE: Store at 2 - 5oC. Freeze/Thawing not recommended.
Derivation: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin).
PERFORMANCE: 500K cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-MHC Class I at 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-mouse IgG:FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 2.26 log10 fluorescent units when compared to a Mouse IgG2a negative control (RDI- MIGG2AX2 $188.00) at a similar concentration.
References: G.S. Eisenbarth, et al, (1980) J Immunol 124:1237-1244. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194. K.L. Rock, (1996) Immunol Today 17: 131-137.
Background: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
For Research Use Only
Also available:
non conjugated cat#RDI-MHC1abm-3, new cat#: 10R-M109a $346.50/100ug
FITC conjugated: cat#RDI-MHC1abm-3FT, new cat#: 61R-M109aFT $412.50/120T
Biotin conjugated: cat#RDI-MHC1abm-3BT, new cat#: 61R-M109aBT $412.50/100ug
PE conjugated: cat#RDI-MHC1abm-3PE, new cat#: 61R-M109aPE $525.00/100T
Monoclonal anti-human MHC Class I (HLA-A, B and C):FITC Conjugated
cat# RDI-MHC1abm-3FT $375.00/120 tests
Presentation: 120 Tests in 0.2ml (0.25mg/ml)50 mM Sodium Phosphate pH 7.5,
100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a
preservative).
Clone: 3F10
Isotype: Mouse IgG2a
Immunogen: Human HSB-2 T cell line
Reactivity: Antibody 3F10 recognizes a monomorphic Class I epitope on the a
chain. Antibody 3F10 (whole molecule) is cytotoxic in the presence of
complement (1).
STORAGE: Store at 2 - 5oC. DO Not Freeze. Protect From Light
Derivation: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. F:P 7.4
PERFORMANCE: 500K cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-MHC Class I/FITC at 1:50 dilution. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.66 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control (RDI- MIGG2AFTX2 $188.00) at a similar concentration. Binding was 95% blocked when pre incubated 10 minutes with 20 ul of 0.5 ug/ml anti-MHC Class I antibody (unconjugated, cat#RDI-MHC1abm-3f $312.00/100ug)
References: G.S. Eisenbarth, et al, (1980) J Immunol 124:1237-1244. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194. K.L. Rock, (1996) Immunol Today 17: 131-137.
Backgorund: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
For Research Use Only
Also available:
non conjugated cat#RDI-MHC1abm-3F $312.00/100ug
FITC conjugated: cat#RDI-MHC1abm-3FT $375.00/120T
Biotin conjugated: cat#RDI-MHC1abm-3BT $375.00/100ug
PE conjugated: cat#RDI-MHC1abm-3PE $500.00/100T
Monoclonal anti-human MHC Class I (HLA-A, B and C):Biotin Conjugated
cat# RDI-MHC1abm-3BT $375.00/100ug
Presentation: 100ug in 0.1ml (1mg/ml)50 mM Sodium Phosphate pH 7.5, 100 mM
Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a
preservative).
Clone: 3F10
Isotype: Mouse IgG2a
Immunogen: Human HSB-2 T cell line
Reactivity: Antibody 3F10 recognizes a monomorphic Class I epitope on the a
chain. Antibody 3F10 (whole molecule) is cytotoxic in the presence of
complement (1).
STORAGE: Store at 2 - 5oC. Freeze/Thawing not recommended.
Derivation: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
PERFORMANCE: 500K cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-MHC Class I/Biotin at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Streptavidin/ R-Phycoerythrin), after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 2.92 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control (RDI- MIGG2aBTX $188.00) at a similar concentration. Binding was 98% blocked when pre incubated 10 minutes with 20 ul of 0.5 ug/ml anti-MHC Class I antibody (unconjugated, cat#RDI-MHC1abm-3 $312.00/100ug)
References: G.S. Eisenbarth, et al, (1980) J Immunol 124:1237-1244. P.J. Bjorkman & P. Parham, (1990) Annu Rev Biochem 59: 253-288. L.D. Barber & P. Parham, (1994) J Exp Med 180: 1191-1194. K.L. Rock, (1996) Immunol Today 17: 131-137.
Backgorund: Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
For Research Use Only
Also available:
non conjugated cat#RDI-MHC1abm-3F $312.00/100ug
FITC conjugated: cat#RDI-MHC1abm-3FT $375.00/120T
Biotin conjugated: cat#RDI-MHC1abm-3BT $375.00/100ug
PE conjugated: cat#RDI-MHC1abm-3PE $500.00/100T
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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