rev:       Ocotber 19, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DATA SHEET: Mouse anti-Human HLA-DO

Catalog#: RDI-HLADO-DOB   $281.00/vial

Package Size: 100ug purified antibody in 0.2ml   (0.5mg/ml) Tris buffer w/0.09%NaN3

Species: mouse

Isotype: mIgG2bk

clone: DOB.L1

Reactivity: Reacts with HLA-DO, a heterodimer formed by DN...and DO subunits in B cells. DN...and DO are the products of the non- classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen- presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.1-2

Uses: -immunofluorescent staining

          -flow cytometry

          -western blot

         -immunoprecipitation

ref: 1. Kropshofer, H., A.B. Vogt, C. Thery, et al. 1998. Increased efficiency in peptide loading of MHC class II molecules through HLA-DO. EMBO J. 11: 2971.

       2. Liljedahl, M., T. Kuwana, W-P. Fung- Leung, et al. 1996. HLA-DO is a lysosomal resident which requires association with HLA-DM for efficient intracellular transport. EMBO J. 15: 4817

Storage: Store at 4 DEG C. For extensive dilutions, add protein containing or stabilizing medium.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive   compounds.

Also available:

FITC conjugated cat#RDI-HLADO-FT  $562.00/vial 100 tests includes matched isotype control

PE conjugated cat#RDI-HLADO-PE      $625.00/vial 100T includes mateched isotype control


FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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