rev: Ocotber 19, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Catalog#: RDI-HLADO-DOB $281.00/vial
Package Size: 100ug purified antibody in 0.2ml (0.5mg/ml) Tris buffer
w/0.09%NaN3
Species: mouse
Isotype: mIgG2bk
clone: DOB.L1
Reactivity: Reacts with HLA-DO, a heterodimer formed by DN...and DO subunits
in B cells. DN...and DO are the products of the non- classical class II genes,
HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the
endoplasmic reticulum and is thereby sorted to lysosomal vesicles during
antigen processing and presentation. It is tightly associated with DM and
it is selectively expressed on antigen- presenting cells, such as B cells,
dendritic cells and thymic epithelial cells. DO can enhance the efficiency
of peptide loading and has been found to stabilize DM at low pH, preserving
its chaperon activity. DO-DM complexes are more efficient than DM in protecting
empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody
has also been reported to be suitable for western blot and
immunoprecipitation.1-2
Uses: -immunofluorescent staining
-flow cytometry
-western blot
-immunoprecipitation
ref: 1. Kropshofer, H., A.B. Vogt, C. Thery, et al. 1998. Increased efficiency in peptide loading of MHC class II molecules through HLA-DO. EMBO J. 11: 2971.
2. Liljedahl, M., T. Kuwana, W-P. Fung- Leung, et al. 1996. HLA-DO is a lysosomal resident which requires association with HLA-DM for efficient intracellular transport. EMBO J. 15: 4817
Storage: Store at 4 DEG C. For extensive dilutions, add protein containing or stabilizing medium.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
Also available:
FITC conjugated cat#RDI-HLADO-FT $562.00/vial 100 tests includes matched isotype control
PE conjugated cat#RDI-HLADO-PE $625.00/vial 100T includes mateched isotype control
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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