rev: January 13, 2000

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

MOUSE MONOCLONAL ANTIBODY TO: HUMAN HLA CLASS Il (DQ) - DISCONTINUED

PRODUCT CODE   RDI-CBL118    $375.00/vial

also available FITC conjugated     cat#RDI-CBL118FT   $375.00/100T

                           PE conjugated:    cat#RDI-CBL118PE   $469.00/100T

Bulk, no BSA, No Azide cat#RDI-CBL118-1XP  $1062.00/1mg  Larger sizes on request

CLONE    SPV-L3

ISOTYPE    IgG2a

IMMUNIZING ANTIGEN   Cell line HG-38

FUSION PARTNER   SP2/0 myeloma cell line

SOURCE

Tissue culture supernatant

PURIFICATION METHOD

Ammonium sulphate precipitation followed by ion exchange chromatography

SPECIFICITY This antibody reacts with HLA Class II DQ antigens (28-33 kDa) and will block strongly the activity of T4+ cytotoxic T cell clones.

APPLICATIONS

*Flow cytometric studies of cells expressing DQ antigen (B lymphocytes and a subpopulation of monocytes).

*Immunohistochemical staining on frozen sections of lymphoid tissue expressing the DQ antigen.

*Immunoprecipitation studies of the DQ antigen.

*Biological blockade studies

REFERENCES

Spits H., et al. Eur. J. Immunol. 14, 299-304 (1984)

Hybridoma 2, 423-437 (1983)

Bontrop, R., et al. Tissue Antigens, 27, 22-31 (1986)

PRESENTATION

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk Quotes Upon Request

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC USE.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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