rev: November 11, 1999

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  anti-RAT and MOUSE ANTIBODIES  

CROSS REACTIVITY WITH HUMAN IG STUDY

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

CROSS-REACTIVITY TEST OF RAT ANTI-MOUSE Ig MAb WITH HUMAN IMMUNOGLOBULIN

Experimental conditions

EIA plates (NUNC, Denmark, Cat. No. 4-69914) were coated overnight at 4°C with 10 µg/ml polyclonal human IgG (Gamma 16, Institut Mérieux, France) in 0.01M borate buffer at pH 9.6. Remaining binding sites were saturated with 0.5% gelatin in PBS 0.1% Tween. MAbs were revealed with peroxidase labeled Rabbit anti-Rat Ig antibodies. All incubations were done for 1 hour at room temperature and washings made with PBS 0.1% Tween. A signal was considered as positive if twice the background O.D. obtained by incubation with P.O. labeled antibody without previous incubation with rat antibodies.

Results

ANTIBODY	Concentration at which a positive signal appears	Cross reaction with Human IgG
LO-MK-1	>15600 ng/ml	NO
LO-MG1-2	<15ng/ml	YES
LO-MG1-13	>1200 ng/ml	YES*
LO-MG2a-2	>62500 ng/ml	NO
LO-MG2a-3	not at 250000 ng/ml	NO
LO-MG2b-2	not at 250000 ng/ml	NO
LO-MG3-7	>15600 ng/ml	NO

*Yes but sometimes can be avoided by careful maniulations

When labeled with peroxidase, these MABs can be used at concentrations of 125-500ng/ml

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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