rev: 5/16/2008
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RAT anti-MOUSE ANTIBODIES
ELISA ASSAY RECOMMENDED PROTOCOLS
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All antibodies are for in vitro research us eonly-not for use in diagnostics.
DETECTION AND QUANTIFICATION OF MOUSE IgM BY ELISA
Sandwich ELISA assays
Option 1 Option 2 Option 3 Option 4
Detector antibodies LO-MM-9 LO-MM-8 LO-MM-8 LO-MK-1*
Capture antibodies LO-MM-3 LO-MM-3 LO-MM-9 LO-MM-3
Standard MADNP-5
Option 1 is recommended________________________________________________________________
Assay conditions
- Coated antibody: LO-MM-3 at 5 µg/ml (in a carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: 5% lyophilized skimmed milk or some other proteins
- Detector antibody:LO-MM-9 conjugated to biotin and then streptavidin-peroxidase (or any other detection system) or LO-MM-8 conjugated to peroxidase
- Normal range of detectable mouse IgM concentrations in this assay: 2 to 0.1 µg/ml
________________________________________________________________
*Remark: Only kappa mouse IgM molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgD BY ELISA
Sandwich ELISA assays
Option 1 Option 2
Detector antibodies LO-MD-8 LO-MK-1*
Capture antibodies LO-MD-6 LO-MD-6
Option 1 is recommended
________________________________________________________________
Assay conditions
- Coated antibody: LO-MD-6 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MD-8 conjugated to biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgD concentrations in this assay:2 to 0.1 µg/ml
_______________________________________________________________
*Remark: Only kappa mouse IgD molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgA BY ELISA
Sandwich ELISA assays
Option 1 Option 2
Detector antibodies LO-MA-7 LO-MK-1*
Capture antibodies LO-MA-10 LO-MA-10
Standards Upon request
Option 1 is recommended
_______________________________________________________________
Assay conditions
- Coated antibody: LO-MA-10 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MA-7 conjugated to biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgA concentrations in this assay:2*0.1 µg/ml
________________________________________________________________
*Remark: Only kappa mouse IgA molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are
given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgE BY ELISA
Sandwich ELISA assays
Option 1 Option 2
Detector antibodies LO-ME-2 LO-MK-1*
Capture antibodies LO-ME-3 LO-ME-3
Option 1 is recommended
_(note: you may be able to use cat#RDI-M1654clb, new cat#: 10R-CD3aHU as a
standard, equal mouse anti-human CD3E (mouse IgE subclass)_______________________________________________________________
Assay conditions
- Coated antibody: LO-ME-3 at 5 µg/ml (in carbonate/bicarbonate buffer pH
9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-ME-2 conjugated to peroxidase or biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgE concentrations in this assay:2 to 0.1 µg/ml
________________________________________________________________
*Remark: Only kappa mouse IgE molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are
given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgG1 BY ELISA
Option 1 Option 2
Detector antibodies LO-MG1-2 LO-MK-1*
Capture antibodies LO-MG1-13 LO-MG1-13
Standard (cat#RDI-MADNP-1 $375.00/1mg)
Option 1 is recommended
________________________________________________________________
Assay conditions
- Coated antibody: LO-MG1-13 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MG1-2 conjugated to peroxidase or biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgG1 concentrations in this assay:2 to 0.1 µg/ml
________________________________________________________________
*Remark: Only kappa mouse IgG1 molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgG2a BY ELISA
Sandwich ELISA assays
Option 1 Option 2 Option 3 Option 4
Detector antibodies LO-MG2a-3 LO-MG2a-2 LO-MG2a-7 LO-MK-1*
Capture antibodies LO-MG2a-7 LO-MG2a-7 LO-MG2a-9 LO-MG2a-9
Standard (cat#RDI-MADNP-2 $375.00/1mg) or $1125.00/5mg)
Option 1 is recommended
________________________________________________________________
Assay conditions
- Coated antibody: LO-MG2a-7and LO-MG2a-9 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MG2a-2 or LO-MG2a-3 or LO-MG2a-7 conjugated to peroxidase or biotin and then streptavidin-peroxidase (or any other detection system)
- Normal range of detectable mouse IgG2a concentrations in this assay:2 to 0.1 µg/ml
________________________________________________________________
*Remark: Only kappa mouse IgG2a molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are
given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgG2b BY ELISA
______________________________________________________________
- LO-MG2b-1 and LO-MK-1 can be used in Sandwich ELISA.
- LO-MG2b-1 coats on plastic plate.
- Standard: ( cat#RDI-LOMADNP-3 $375.00/1mg $1125.00/5mg)
________________________________________________________________
Assay conditions
- Coated antibody: LO-MG2b-1 at 10 µg/ml (in carbonate/bicarbonate buffer
pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MK-1 conjugated to peroxidase or biotin and then
streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgG2b concentrations in this assay:2 to
0.1µg/ml
________________________________________________________________
Remark: Only kappa mouse IgG2b molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are
given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgG3 BY ELISA
Option 1 Option 2
Detector antibodies LO-MG3-7 LO-MK-1*
Capture antibodies LO-MG3-13 LO-MG3-13
Standard (cat#RDI-LOMADNP-4 $375.00/1mg $1125.00/5mg)
Option 1 is recommended
________________________________________________________________
Assay conditions
- Coated antibody: LO-MG3-13 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MG3-7 conjugated to peroxidase or biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgG3 concentrations in this assay:2 to 0.1
µg/ml
________________________________________________________________
Remark: Only kappa mouse IgG3 molecules are detected. In normal serum, mouse Ig molecules bearing kappa light chain represent about 95% of the total.
For each batch of monoclonal antibodies, all details of the ELISA controls are
given in the quality control sheet.
DETECTION AND QUANTIFICATION OF MOUSE IgK BY ELISA
- LO-MK-1 and LO-MK-2 do not bind to the same epitope(s) of mouse IgK.
- LO-MK-2 coats very well on plastic plate.
- Standards: upon request
________________________________________________________________
Assay conditions
- Coated antibody: LO-MK-2 at 5 µg/ml (in carbonate/bicarbonate buffer pH 9.5)
- Saturating protein: lyophilized skimmed milk or some other proteins
- Detector antibody: LO-MK-1 conjugated to peroxidase or biotin and then streptavidin-peroxidase(or any other detection system)
- Normal range of detectable mouse IgK concentrations in this assay:2 to 0.6 µg/ml
________________________________________________________________
For each batch of monoclonal antibodies, all details of the ELISA controls are given in the quality control sheet.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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