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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARM-4


IMMUNOGEN  SUBSTANCE NAME : IgM (Polyclonal rat IgM, IR968) (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C


IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c


IMMORTAL CELL PARTNER DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY (2)

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARM-4 HYBRIDOMA

NAME FOR PRODUCT : MARM-4 MONO Ab

ICDB NUMBER: 3032665

AVIDITY: on rat IgM: 3.5 x 109 M-1 (Cf. avidity sheet, for more details).



REACTIVITY (2):Rat mu heavy chain of immunoglobulin (determined by immunodot).

MARM-4 and MARM-7 do not bind to the same epitope.

MARM-4 and MARM-7 label the same percentage of IgM rat splenocytes by fluorocytometric analyses.


CROSS-REACTIVITY: MARM-4 doesn't bind to mouse Ig (every isotype) and rabbit IgG.

APPLICATIONS : Cf REACTIVITY

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE, BIOTIN, TRITC AND FITC

- CANNOT BE USED FOR CELL COUNTING USING A DIRECT METHOD OF IMMUNOFLUORESCENCE

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of rat IgM (solid Sepharose 4B CNBr act.)

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC


LYOPHILIZATION: Yes, with a possible low denaturation.


REFERENCES

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology, 1974,4:44-48.

(2) Cormont F., Manouvriez P., De Clercq L. & Bazin H. The use of rat monoclonal antibodies to characterize, quantify and purify polyclonal or monoclonal mouse IgM. Meth. in Enzymology, 1986,121,622-631.


Selected references of scientific publications in which MARM-4 MAb was used


Platteau B. et al. Int. J. Radiat. Biol., 1989, 55:14; Soares M. et al. Transplantation Proceedings, 1992,24:451-452; Castedo M. et al. Int. Immunol. 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Latinne et al. Immunol. Rev. 1994, 141: 95-125; Reding et al. Transplant Immunol. 1994, 2: 231-237; Soares et al., Transplantation 1994, 57: 1003-1009; Soares et al., Xenotransplantation 1994, 1: 118-124; Soares et al. Transplant. Proc. 1994, 26: 1357-1359; Scheringa M. et al., Transplantation 1995, 60: 1350-1353; Soares et al., Transplant. Proc. 1995, 27: 282-285.


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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