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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARL-15

IMMUNOGEN  SUBSTANCE NAME : Lambda light chain (RH58, IR31) (1,2,3)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER DESIGNATION : PAI-O (PAI-O is a published cell line of BALB/c origin)

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARL-15 HYBRIDOMA

NAME FOR PRODUCT : MARL-15 MONO Ab

ICDB NUMBER: 3046771

REACTIVITY: Rat lambda light chain of immunoglobulin (determined by immunodot)

AVIDITY: on rat IgG1 lambda: 2.6 x 1010 M-1(Cf. avidity sheet, for more details).

APPLICATIONS : Cf REACTIVITIES

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE

- CAN BE LABELLED WITH FITC AND BIOTIN

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Rat Ig of the lambda type

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC

LYOPHILIZED: not tested

REFERENCES

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

(2) Querinjean P., Bazin H., Starace V., Beckers A., Deckers C. & Heremans J.F. Lambda light chains in rat immunoglobulins. Immunochemistry 1973,10:653-654

(3) Burtonboy G., Bazin H. & Delferrière N. Rat hybridoma antibodies against canine parvovirus. Archives of Virology 1982,71:291-302

Selected references of scientific publications in which MARL-15 MAb was used

Soares et al., Transplant. Proc. 1995, 27: 282-285

For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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