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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARK-3


IMMUNOGEN  SUBSTANCE NAME : Kappa L-chain of the IgK-1a allotype (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR  GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARK-3 HYBRIDOMA

NAME FOR PRODUCT : MARK-3 MONO Ab

ICDB NUMBER: 3032661


REACTIVITY : Rat Kappa light chain of Ig (allotype IgK-1a) (determined by immunodot) CROSS-REACTS WEAKLY IN IMMUNOFLUORESCENCE, ELISA AND RIA WITH RAT IgK-1b KAPPA LIGHT CHAIN OF IMMUNOGLOBULIN.


AVIDITY: on rat kappa 1a IgD: 4.8 x 109 M_1(Cf. avidity sheet, for more details).


APPLICATIONS : Cf REACTIVITIES

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of rat IgK-1a allotype Ig of the Kappa type (Solid phase Sepharose 4B CNBr act.)

- CAPTURE IN ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE, BIOTIN, ALCALINE PHOSPHATASE, FITC AND TRITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CANNOT BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH FITC (NOT GOOD WHEN LABELLED WITH PEROXIDASE)


LYOPHILIZATION: Yes.

REFERENCE

(1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes.    
      Transpl. Proc. 1983,15:1685-1686


Selected references of scientific publications in which MARK-3 MAb was used

Bazin H. et al. J. Immunol. Meth. 1984,71:9-16

Bazin H. et al. J. Immunol. Meth. 1984,66:261-269

Manouvriez P. et al. Immunobiology 1986, 173, 308-309.

Bazin H., Malache J.M. J. Immunol. Meth., 1986, : 19-24.

Manouvriez P. et al. Mol. Immunol., 1985, 22:1201-1208.

Chavez V., et al. Int. Arch. Allergy Immunol., 1992, 97:330-336.

Vanhove B., Bazin H. Immunology 1993, 79: 580-586.

Fernandes I. et al. Brazilian J. Med. Biol. Res. 1994, 27: 2599-2606

Soares et al., Transplant. Proc. 1995, 27: 282-285


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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