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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARK-1


IMMUNOGEN  SUBSTANCE NAME : Kappa L-chain

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER DESIGNATION : Sp2/O


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARK-1 HYBRIDOMA

NAME FOR PRODUCT : MARK-1 MONO Ab

ICDB NUMBER: 3032660


REACTIVITY: Rat Kappa light chain of Ig (determined by immunodot)


AVIDITY: on rat IgD kappa: 1 x 109M-1 (Cf. avdity sheet, for more details).


CROSS-REACTIVITY: MARK-1 does not cross react with human IgG coated in ELISA plate. No specific binding on human platelets.


APPLICATIONS : Cf REACTIVITY

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of rat Ig of the Kappa type (Solid phase Sepharose 4B CNBr act.)

- CAPTURE ELISA: MEDIUM BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE, BIOTIN, FITC AND TRITC

- CAN BE LABELLED WITH IODINE (4) AND ALKALINE PHOSPHATASE

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CANNOT BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC


LYOPHILIZATION: Yes.


REFERENCES

Selected references of scientific publications in which MARK-1 MAb was used

Bazin H. et al. J. Immunol. Methods 1984,66:261-269; Bodeus M. et al. J. Immunol. Methods 1985,79:1-6; Khalife J. et al. J. Immunol. 1985,135:2780-2784; Bazin H., Malache J.M. J. Immunol. Meth., 1986, : 19-24; Bazin H. et al. Meth. Enzymol., 1986, 121:638-652; Manouvriez P. et al. Immunobiology 1986, 173, 308-309; Pelletier L. et al. Clin. Exp. Immunol., 1988, 71: 336-342; Castedo M. et al. Int. Immunol. 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Saoudi A. et al. Int. Immunol. 1993, 5: 1559-1567; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Gmür, R. & Guggenheim, B. J. Dent. Res. 1994, 73: 1421-1428; Reding et al. Transplant Immunol. 1994, 2: 231-237; Lepault F. et al. Eur. J. Immunol. 1995, 25: 1502-1507; Scheringa M. et al., Transplantation 1995, 60: 1350-1353; Soares et al., Transplant. Proc. 1995, 27: 282-285

For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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