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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARG2b-8


IMMUNOGEN

SUBSTANCE NAME : IgG2b (IR863) (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C


IMMUNOCYTE DONOR

GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c


IMMORTAL CELL PARTNER

DESIGNATION : Sp2/O


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARG2b-8 HYBRIDOMA

NAME FOR PRODUCT : MARG2b-8 MONO Ab

ICDB NUMBER: 3032652


REACTIVITY : Rat gamma 2b heavy chain of immunoglobulin (determined by immunodot)

The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations.

Increasing of the first or second antibody up to an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.


AVIDITY: on rat IgG2b: 4.7 x 109M-1 (Cf. avidity sheet, for more details).


APPLICATIONS : Cf REACTIVITY

- CAPTURE ELISA: WEAK BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE, AND BIOTIN

- CANNOT BE LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS (2,3)

- CROSS-REACTS NON SPECIFICALLY WITH NEARLY 15% OF HUMAN LYMPHOCYTES (as detected by FACScan) AND WITH HUMAN MONOCYTES (> 35%)

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of rat IgG2b (Solid phase Sepharose 4B CNBr act.)

- CANNOT BE COATED ON NITROCELLULOSE (DOT-ELISPOT)


LYOPHILIZATION: Yes, with a possible low denaturation.


REFERENCES

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

(2) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416

(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.


Selected references of scientific publications in which MARG2b-8 MAb was used


Castedo M. et al. Int. Immunology 1993, 5: 1569-1576

Soares M. et al. Transplantation, 1993, 56: 1427-1433

Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8

Reding et al. Transplant Immunol. 1994, 2: 231-237


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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