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MOUSE anti-RAT ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : MARG2b-3, new cat#: 10R-G102a $656.25/1mg
IMMUNOGEN SUBSTANCE NAME : IgG2b (IR863) (1)
GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse
STRAIN : BALB/c
IMMORTAL CELL PARTNER DESIGNATION : Sp2/O
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY (2,3)
CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1
NAME FOR CELL LINE : MARG2b-3 HYBRIDOMA
NAME FOR PRODUCT : MARG2b-3 MONO Ab
ICDB NUMBER: 3000195
REACTIVITY : Rat gamma 2b heavy chain of immunoglobulin (determined by immunodot) The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody up to an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.
AVIDITY: on rat IgG2b: 3 x 109M-1 (Cf. avidity sheet, for more details).
APPLICATIONS: Cf. REACTIVITIES (4)
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE LABELLED WITH BIOTIN, PEROXIDASE AND FITC
- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)
- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC
- CANNOT BE FROZEN AND THAWED MANY TIMES. SUSCEPTIBLE TO BE DENATURATED BY SUCH A TREATMENT.
LYOPHILIZATION: not tested
REFERENCES
(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48
(2) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416
(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.
(4) Bazin H., Malache JM. Rat (and mouse) monoclonal antibodies. V. A simple automated technique of antigen purification by immunoaffinity chromatography. J. Immunol. Meth., 1986, 88:19-24
Selected references of scientific publications in which MARG2b-3 was used:
Soares et al., Transplant. Proc. 1995, 27: 282-285
For more information, see: "Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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