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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARG2a-1

IMMUNOGEN SUBSTANCE NAME : IgG2a (Polyclonal antibodies anti horse spleen ferritin, IR33 (1,2)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER

DESIGNATION : PAI-O (PAI-O IS A PUBLISHED CELL LINE of BALB/c ORIGIN)

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARG2a-1 HYBRIDOMA

NAME FOR PRODUCT : MARG2a-1 MONO Ab

ICDB NUMBER: 3003907

AVIDITY: on rat IgG2a: 2.1 x 109M-1 Cf. avidity sheet, for more details.


REACTIVITY : Rat gamma 2a heavy chain of immunoglobulin (determined by immunodot) (2)

The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations.

Increasing of the first or second antibody up to an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.


APPLICATIONS : Cf REACTIVITIES

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Rat IgG2a (Solid phase Sepharose 4B CNBr act.)

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE LABELLED WITH FITC, BIOTIN, PEROXIDASE

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC

- CANNOT BE FROZEN AND THAWED MANY TIMES. SUSCEPTIBLE TO BE DENATURED BY SUCH A TREATMENT.


LYOPHILIZATION: Yes, with a possible low denaturation.


REFERENCES

(1) Bazin H., Beckers A., Deckers C. & Moriame M. Transplantable immunoglobulin-secreting tumours in rats.V.Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in the LOU/Wsl rats. J. Nat. Cancer Inst. 1973,51:1359-1361

(2) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48


Selected references of scientific publications in which MARG2a-1 was used:

Castedo M. et al. Int. Immunology, 1993, 5: 1569-1576;Soares M. et al. Transplantation 1993, 56: 1427-1433;Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8;Reding et al. Transplant Immunol. 1994, 2: 231-237;Routledge E.G. et al. Transplantation 1995, 60: 847-853;Soares et al., Transplant. Proc. 1995, 27: 282-285


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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