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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

IMMUNOGEN  SUBSTANCE NAME : IgG1 (IR27, IR31) (1) (2)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARG1-2 HYBRIDOMA

NAME FOR PRODUCT : MARG1-2 MONO Ab

ICDB NUMBER: 3003906

REACTIVITY : Rat gamma 1 heavy chain of immunoglobulin (determined by immunodot) (2)

The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations.

Increasing of the first or second antibody up to an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

AVIDITY: on rat IgG1: 1 x 109M-1  Cf. avidity sheet, for more details.

APPLICATIONS : Cf REACTIVITY

- CAN BE LABELLED WITH BIOTIN, FITC, PEROXIDASE

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of rat IgG1 (solid phase Sepharose 4B CNBr act.)

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC

LYOPHILIZATION: not tested

REFERENCES

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48

(2) Querinjean P., Bazin H., Starace V., Beckers A., Deckers C. & Heremans J.F. Lambda light chains in rat immunoglobulins. Immunochemistry 1973,10:653-654

Selected references of scientific publications in which MARG1-2 was used:

Castedo M. et al. Int. Immunology, 1993, 5: 1569-1576

Soares M. et al. Transplantation 1993, 56: 1427-1433

Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8

Yamada M., et al. Immunology 1993, 78 : 298-302.

Reding et al. Transplant Immunol. 1994, 2: 231-237

Soares et al., Transplant. Proc. 1995, 27: 282-285

For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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