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MOUSE anti-RAT ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : MARE-1, new cat#: 10R-I106a $656.25/1mg
IMMUNOGEN SUBSTANCE NAME : IgE (IR162, IR410) (1)
GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMUNOCYTE DONORGENUS SPECIES : Mus musculus - mouse
STRAIN : BALB/c
IMMORTAL CELL PARTNER DESIGNATION : Sp2/O
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1
NAME FOR CELL LINE : MARE-1 HYBRIDOMA
NAME FOR PRODUCT : MARE-1 MONO Ab
ICDB NUMBER: 3032636
REACTIVITY: Rat epsilon heavy chain of immunoglobulin (C epsilon 3) (4)
(determined by immunodot)
AVIDITY: On rat IgE: 4 x 109M-1 (Cf. avidity sheet, for more details).
APPLICATIONS : Cf REACTIVITY
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS (2,3,4,5,6)
- CAN BE LABELLED WITH IODINE (2,3), BIOTIN, FITC AND PEROXIDASE
- CAN USED FOR CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE USED IN WESTERN BLOT ANALYSIS: binds to rat IgE in denaturated and non denaturated conditions.
- SUITABLE FOR DEGRANULATION OF RAT MAST CELLS (positive reaction in PCA)
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Rat IgE (Solid phase Sepharose 4B CNBr act.)
- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)
- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH FITC
LYOPHILIZATION: Yes, with a possible low denaturation.
REFERENCES
(1) Bazin H., Querinjean P., Beckers A., Heremans J.F. & Dessy F.Transplantable immunoglobulin-secreting tumours in rats.IV.Sixty-three IgE-secreting immunocytoma tumours. Immunology 1974,26:713-723; (2) Manouvriez P., Ravoet A.M. & Bazin H. Fc epsilon receptors on rat B- lymphocytes: specificity and binding kinetics. Mol. Immunology 1985,22:1201-1208; (3) Manouvriez P. & Bazin H. In vivo kinetics and nature of rat IgE- bearing lymphocytes after IgE stimulation. J. Immunol. 1984,133:3274- 3281; (4) Rousseaux-Prevost R., Rousseaux J., Bazin H. Studies of the IgE binding sites to rat mast cell receptor with proteolytic fragments and with a monoclonal antibody directed against epsilon heavy chain: evidence that the combining sites are located in the C epsilon 3 domain. Molecular Immunology 1987, 24, 187-196; (5) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416; (6) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.
Selected references of scientific publications in which MARE-1 MAb was used
Manouvriez P.et al. Ann. Inst. Pasteur, 1985, 136C:187-193; Rousseaux-Prevost R., et al. Mol. Immunol., 1987, 24:187-196; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8;Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Yamada M., et al. Immunology 1993, 78 : 298-302; Soares et al., Transplant. Proc. 1995, 27: 282-285
For more information, see: "Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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