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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARD-3


IMMUNOGEN  SUBSTANCE NAME : IgD (IR731) (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER  DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARD-3 HYBRIDOMA

NAME FOR PRODUCT : MARD-3 MONO Ab

ICDB NUMBER: 3032634


REACTIVITY : Rat delta heavy chain of immunoglobulin (determined by immunodot)

AVIDITY: on rat IgD: 1.1 x 1010M-1

Cf. avidity sheet, for more details.


APPLICATIONS : Cf REACTIVITY

- CAN BE USED FOR IMMUNOPURIFICATION OF RAT IgD PROTEIN (Solid phase Sepharose 4B CNBr act.)

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE LABELLED WITH FITC, TRITC AND BIOTIN

- CAN BE USED IN IMMUNOFLUORESCENCE

- CAPTURE ELISA: GOOD BINDING ON PLASTICS.

- CAN BE USED IN WESTERN BLOT ANALYSIS (denaturated and non denaturated conditions)

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY (purified or labelled with FITC)


LYOPHILIZATION: not tested

REFERENCE

(1) Bazin H., Beckers A., Urbain-Vansanten G., Pauwels R., Bruyns C., Tilkin A.F., Platteau B. & Urbain J. Transplantable IgD immunoglobulin- secreting tumours in rats. J. Immunol. 1978,121,2077-2082

Selected references of scientific publications in which MARD-3 MAb was used

Manouvriez P. & Bazin. J. Immunol. 1984,133,3274- 3281

Chavez V. et al. Int. Arch. Allergy Immunol., 1992,97:330-336.

Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8

Soares M. et al. Transplantation 1993, 56: 1427-1433

Vanhove B., Bazin H. Immunology 1993, 79: 580-586.

Latinne et al. Immunol. Rev. 1994, 141: 95-125

Soares et al., Transplant. Proc. 1995, 27: 282-285

For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.



FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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