rev: November 13, 1999

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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARA-2


IMMUNOGEN   SUBSTANCE NAME : IgA (IR1060, IR22, IR699) (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMUNOCYTE DONOR  GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER

DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARA-2 HYBRIDOMA

NAME FOR PRODUCT : MARA-2 MONO Ab

ICDB NUMBER: 3042726

REACTIVITY : Rat alpha heavy chain of immunoglobulin (determined by immunodot)

AVIDITY: on rat IgA: 4.3 x 108M-1 Cf. avidity sheet, for more details.


APPLICATIONS : Cf REACTIVITY

- CAN BE LABELLED WITH BIOTIN AND PEROXIDASE

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED FOR CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE USED IN WESTERN BLOT ANALYSIS : binds to rat IgA in denaturated and non denaturated conditions

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE OR FITC

- CANNOT BE USED FOR IMMUNOPURIFICATION


LYOPHILIZATION: Yes, with a possible low denaturation.


REFERENCE

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.



FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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