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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : MARA-1

IMMUNOGEN  SUBSTANCE NAME : IgA (IR1060, IR22, IR699) (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C


IMMUNOCYTE DONOR  GENUS SPECIES : Mus musculus - mouse

STRAIN : BALB/c

IMMORTAL CELL PARTNER  DESIGNATION : Sp2/O

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Mouse kappa IgG1

NAME FOR CELL LINE : MARA-1 HYBRIDOMA

NAME FOR PRODUCT : MARA-1 MONO Ab

ICDB NUMBER: 3042857

REACTIVITY : Rat alpha heavy chain of immunoglobulin (determined by immunodot)

AVIDITY: on rat IgA: 1.1 x 1010M-1

Cf. avidity sheet, for more details.

APPLICATIONS : Cf REACTIVITY

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE LABELLED WITH BIOTIN, PEROXIDASE AND FITC

- CAN BE USED FOR CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE USED IN WESTERN BLOT ANALYSIS (only in non denaturated conditions)

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY (Solid phase Sepharose 4B CNBr act.)

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

LYOPHILIZATION: Yes, possibly with a low denaturation.

REFERENCE

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48.

Selected references of scientific publications in which MARA-1 MAb was used


Soares et al. Transplantation, 1993, 56: 1427-1433

Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8

Vanhove B. & Bazin H. Immunology 1993, 79: 580-586.

Latinne D., et al. Immunol. Rev. 1994, 141: 95-125

Soares et al., Transplant. Proc. 1995, 27: 282-285

For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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