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  MOUSE anti-RAT ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-RK1b-2

IMMUNOGEN  SUBSTANCE NAME : Kappa L-chain of the IgK-1b allotype (1)

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : OKA

IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/

IMMORTAL CELL PARTNER

DESIGNATION : non secreting LOU/C rat IR983F fusion line (2)

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a

NAME FOR CELL LINE : LO-RK1b-2 HYBRIDOMA

NAME FOR PRODUCT : LO-RK1b-2 MONO Ab

ICDB NUMBER: 3000338

REACTIVITY : Rat Kappa light chain of immunoglobulin of the IgK-1b allotype   (determined by immunodot)

No cross-reaction with rat kappa light chain of the IgK-1a allotypes

APPLICATIONS : Cf REACTIVITY

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Rat IgK-1b Kappa Ig (Solid phase Sepharose 4B CNBr act.)(3)

- CAN BE LABELLED WITH BIOTIN

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE


LYOPHILIZATION: not tested

REFERENCES

1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes. Transpl. Proc. 1983,15:1685-1686

(2) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981, Pergamon Press Oxford and N.Y., pp. 615-618

(3) Bazin H., Xhurdebise L.M., Burtonboy G., Lebacq A.M., De Clercq L. & Cormont F. Rat monoclonal antibodies.I.Rapid purification from in vitro culture supernatants. J. Immunol. Meth. 1984,66:261-269

Selected references of scientific publications in which LO-RK1b-2 was used

Vanhove B., Bazin H. Immunology 1993, 79: 580-586.

Latinne D., et al. Immunol. Rev. 1994, 141: 95-125

Soares et al., Transplant. Proc. 1995, 27: 282-285


For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies". H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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