IMMUNOGEN SUBSTANCE NAME : Kappa L-chain of the IgK-1b allotype (1)
GENUS SPECIES : Rattus norvegicus - rat
STRAIN : OKA
IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion
line (2)
IMMUNOREACTIVE CELL AND PRODUCTS
CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a
NAME FOR CELL LINE : LO-RK1b-1 HYBRIDOMA
NAME FOR PRODUCT : LO-RK1b-1 MONO Ab
ICDB NUMBER: 3000337
REACTIVITY : Rat Kappa light chain of immunoglobulin of the IgK-1b allotype
(determined by immunodot)
No cross-reaction with rat kappa light chain of the IgK-1a allotype
APPLICATIONS : Cf REACTIVITY
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE LABELLED WITH PEROXIDASE
- CAN BE LABELLED WITH BIOTIN
- CAN BE LABELLED WITH FITC
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Rat IgK-1b Kappa Ig (Solid phase Sepharose 4B CNBr act.) (3)
LYOPHILIZATION: not tested
REFERENCES
(1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes. Transpl. Proc. 1983,15:1685-1686
(2) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981, Pergamon Press Oxford and N.Y., pp. 615-618
(3) Bazin H., Xhurdebise L.M., Burtonboy G., Lebacq A.M., De Clercq L. & Cormont F. J. Immunol. Rat monoclonal antibodies.I.Rapid purification from in vitro culture supernatants. Meth. 1984,66:261-269
For more information, see: "Rat Hybridomas and Rat Monoclonal Antibodies".
H. BAZIN (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515
pages
FOR RESEARCH USE ONLY
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