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RAT anti-MOUSE ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : LO-MM-9, new cat#: 10R-I124a $484.00/1mg
IMMUNOGEN SUBSTANCE NAME : purified IgM (Mouse polyclonal IgM) from
BALB/c mice
GENUS SPECIES : Mus musculus - mouse
IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER
DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG2a, allotype IgK-1a
NAME FOR CELL LINE : LO-MM-9 HYBRIDOMA
NAME FOR PRODUCT : LO-MM-9 MONO Ab
ICDB NUMBER: 3039019
REACTIVITY : Mu heavy chain of Mouse immunoglobulin (2) (determined by
immunodot), positively tested on BALB/c and C57Bl/7 mouse sera. It binds to an
epitope in the Cµ3 domain.
CROSS-REACTIVITY: does not bind to human myeloma of IgM, IgG, IgA, IgD, IgE
isotypes. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog,
swine, baboon IgG (ELISA test).
AVIDITY: on mouse IgM: 6.8 x 108 M-1 by BIACORE (Pharmacia)*
Cf. avidity sheet, for more details
APPLICATIONS : Cf REACTIVITY
- CAN BE LABELLED WITH IODINE (2), FITC and BIOTIN
- CANNOT BE LABELLED WITH PEROXIDASE (use LO-MM-8)
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgM (Solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.
- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)
- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY (purified) (3)
LYOPHILIZATION: Yes, but with partial denaturation.
REFERENCES
(1) Bazin H. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618; (2) Cormont F., Manouvriez P., De Clercq L., Bazin H. Meth. in Enzymol. 1986, 121: 622-631; Bazin H., Malache J.M. J. Immunol. Meth. 1986, 88: 19-24; (3) Latinne et al. Immunol. Rev. 1994, 141: 95-125
Selected references of scientific publications in which LO-MM-9 MAb was used
Bendelac A. et al. J. Immunol., 1988, 141:2625-2628; Cerny A. et al. Transplantation, 1988, 45: 1111-1113.
Cerny A. et al. J. Virol., 1988, 62: 1803-1807; Cormont F. et al. Meth. Enzymol., 1986, 121: 622-631; Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78; Latinne D. et al. Immunol. Rev. 1994, 141: 95-125
For more information, see: Rat Hybridomas and Rat Monoclonal
FOR RESEARCH ONLY
* Kindly determined by Th. Delaunay (
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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