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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-MM-3

IMMUNOGEN  SUBSTANCE NAME : purified IgM (Mouse polyclonal IgM) from BALB/c mice

GENUS SPECIES : Mus musculus - mouse

IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgM, allotype IgK-1a.

NAME FOR CELL LINE : LO-MM-3 HYBRIDOMA

NAME FOR PRODUCT : LO-MM-3 MONO Ab

ICDB NUMBER: 3003924


REACTIVITY : Mu heavy chain of Mouse immunoglobulin (2) (determined by immunodot)


AVIDITY: on mouse IgM: 2 x 108 M-1 Cf. avidity sheet, for more details


CROSS-REACTIVITY: does not bind to rabbit, swine and human IgG. Weak reaction with chicken, goat, sheep, bovine, horse, dog, baboon IgG and with human IgM (ELISA test).


APPLICATIONS : Cf REACTIVITY

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE LABELLED WITH TRITC

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgM (Solid phase Sepharose 4B CNBr act.)


LYOPHILIZATION: not tested


REFERENCES

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618

(2) Cormont F., Manouvriez P., De Clercq L., Bazin H. The use of rat monoclonal antibodies to characterize, quantify and purify polyclonal or monoclonal mouse IgM. Meth. in Enzymol. 1986,121:622-631


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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