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RAT anti-MOUSE ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : LO-MK-2, new cat#: 61R-K103aBT $525.00/1mg
IMMUNOGEN SUBSTANCE NAME : purified IgG kappa from BALB/c mice
GENUS SPECIES : Mus musculus - mouse
IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line
(1)
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a
NAME FOR CELL LINE : LO-MK-2 HYBRIDOMA
NAME FOR PRODUCT : LO-MK-2 MONO Ab
ICDB NUMBER: 3000335
REACTIVITY: Kappa Light Chain of Mouse Immunoglobulin (determined by
immunodot), positively tested on BALB/c and C57Bl/7 mouse sera. LO-MK-1 and
LO-MK-2 do not bind to the same epitope.
CROSS-REACTIVITY: does not cross-react with chicken, rabbit, goat, horse and
baboon human IgG and with human IgM. Cross-react with dog, sheep, swine and
bovine IgG (ELISA test), probably by their kappa light chain, but not tested.
AVIDITY: on mouse kappa IgM: 2 x 108 M-1
on mouse kappa IgG1: 1 x 109 M-1
on mouse kappa IgG2a:3 x 109 M-1
on mouse kappa IgG2b:2 x 109 M-1
on mouse kappa IgG3: 2 x 109 M-1
APPLICATIONS: Cf REACTIVITY
- LABELLED SECOND ANTIBODY, but less efficient than LO-MK-1
- CAN BE LABELLED WITH PEROXIDASE, FITC AND BIOTIN
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of Mouse Kappa Ig (Solid phase Sepharose 4B CNBr act.)
LYOPHILIZATION: Yes.
REFERENCE
(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618
Selected references of scientific publications in which LO-MK-2 MAb was used:
Delaunay T. et al. J. Immunol. Methods, 1990, 131: 33-39.
Vanhove B., Bazin H. Immunology 1993, 79: 580-586.
For more information, see: Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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