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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-MK-1

IMMUNOGEN  SUBSTANCE NAME: purified mouse IgG1 kappa from BALB/c mice

IMMUNOGEN TISSUE SOURCE : serum

IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat

STRAIN : OKA inbred strain


IMMORTAL CELL PARTNER

DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG2a, allotype IgK-1b

NAME FOR CELL LINE : LO-MK-1 HYBRIDOMA

NAME FOR PRODUCT : LO-MK-1 MONO Ab

ICDB NUMBER: 3000334



REACTIVITY: Mouse kappa light chain of immunoglobulin (determined by immunodot), positively tested on BALB/c and C57Bl/7 mouse sera.

LO-MK-1 and LO-MK-2 do not bind to the same epitope.


CROSS-REACTIVITY: does not cross-react with chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon, and human IgG and with human IgM (ELISA test).


AVIDITY: on mouse IgG2a kappa: 3 x 109 M-1   Cf. avidity sheet, for more details


APPLICATIONS: Cf REACTIVITY

- CAN BE LABELLED WITH PEROXIDASE

- CAN BE LABELLED WITH FITC

- CAN BE LABELLED WITH BIOTIN

- CAPTURE ELISA: LOW BINDING ON PLASTICS

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CANNOT BE USED FOR IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse Kappa Ig (NO ELUTION at pH 2.8).

- CAN BE USED IN IMMUNOHISTOLOGY (as second antibody labelled with peroxidase or FITC) (2)


LYOPHILIZATION: Yes, with a possible low denaturation.


REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618

(2) Latinne et al. Immunol. Rev. 1994, 141: 95-125


Selected references of scientific publications in which LO-MK-1 MAb was used:

Delaunay T. et al. J. Immunol. Methods, 1990, 131: 33-39.

Denis O. et al. Internat. Immunol., 1993, 5 (1) : 71-78.


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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