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RAT anti-MOUSE ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : LO-MG2b-2, new cat#: 10R-I118a $484.00/1mg
IMMUNOGEN SUBSTANCE NAME : purified IgG from BALB/c mice
GENUS SPECIES : Mus musculus - mouse
IMMUNOCYTE DONORGENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER DESIGNATION : Non secreting LOU/C rat IR983F fusion line
(1)
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Rat kappa IgG1, allotype IgK-1a
NAME FOR CELL LINE : LO-MG2b-2 HYBRIDOMA
NAME FOR PRODUCT : LO-MG2b-2 MONO Ab
ICDB NUMBER: 3000329
REACTIVITY : Mouse Gamma2b Heavy Chain of Immunoglobulin (determined by
immunodot), positively tested on BALB/c and C57Bl/7 mouse sera.
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.
CROSS-REACTIVITY: does not bind to chicken, rabbit, goat, sheep, bovine, horse,
dog, swine, baboon IgG and human IgG or IgM (ELISA test).
AVIDITY: on mouse IgG2b: 1 x 1010 M-1
Cf. avidity sheet, for more details
APPLICATIONS: Cf REACTIVITY
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgG2b (solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.
- CAN BE LABELLED WITH FITC, BIOTIN AND PEROXIDASE
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAPTURE ELISA: LOW BINDING ON PLASTICS
- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)
LYOPHILIZATION: Yes, possibly with a low denaturation.
REFERENCE
(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed. 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615-618.
Selected references of scientific publications in which LO-MG2b-2 MAb was used
Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78.
El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76
Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182
For more information, see: Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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