rev: November 11, 1999

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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-MG2b-1

IMMUNOGEN  SUBSTANCE NAME : polyclonal IgG from BALB/c mice

GENUS SPECIES : Mus musculus - mouse


IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMORTAL CELL PARTNER DESIGNATION : Non secreting LOU/C rat IR983F fusion line (1)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat lambda IgG1

NAME FOR CELL LINE : LO-MG2b-1 HYBRIDOMA

NAME FOR PRODUCT : LO-MG2b-1 MONO Ab

ICDB NUMBER: 3003931


REACTIVITY : Mouse Gamma2b Heavy Chain of Immunoglobulin (determined by immunodot)

The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.


CROSS-REACTIVITY: does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test).


AVIDITY: on mouse IgG2b: 3 x 108 M-1   Cf. avidity sheet, for more details.

APPLICATIONS : Cf REACTIVITIES

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgG2b (solid phase Sepharose 4B CNBr act.) - capacity and conditions of elution: see data sheet.

- CAN BE LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAPTURE ELISA: MIDDLE BINDING ON PLASTICS


LYOPHILIZATION: not tested


REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed. 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615-618.


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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