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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-MG2a-3

IMMUNOGEN  SUBSTANCE NAME : purified IgG from BALB/c mice

GENUS SPECIES : Mus musculus - mouse

IMMUNOCYTE DONOR  GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat kappa IgG2a, allotype IgK-1a

NAME FOR CELL LINE : LO-MG2a-3 HYBRIDOMA

NAME FOR PRODUCT : LO-MG2a-3 MONO Ab

ICDB NUMBER: 3000328

REACTIVITY : Mouse Gamma2a Heavy Chain of Immunoglobulin (determined by immunodot)

The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.

CROSS-REACTIVITY: does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine and baboonIgG and human IgG or IgM (ELISA test).

AVIDITY: on mouse IgG2a: 3 x 109 M_1    Cf. avidity sheet, for more details.

APPLICATIONS: Cf REACTIVITY

- CAN BE LABELLED WITH BIOTIN AND PEROXIDASE

- CANNOT BE EASILY LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAPTURE ELISA: LOW BINDING ON PLASTICS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgG2a (solid phase Sepharose 4B CNBr act.) - capacity: see data sheet.

LYOPHILIZATION: not tested

REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th Colloquium 1981 Pergamon Press, Oxford and N.Y., pp. 615-618.

Selected references of scientific publications in which LO-MG2a-3 MAb was used

Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182

Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78.

For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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