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RAT anti-MOUSE ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : LO-MG1-2, new cat#: 10R-I109c $484.00/1mg
IMMUNOGEN SUBSTANCE NAME : purified mouse IgG1 from BALB/c mice
GENUS SPECIES : Mus musculus - mouse
IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line
(1)
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a
NAME FOR CELL LINE : LO-MG1-2 HYBRIDOMA
NAME FOR PRODUCT : LO-MG1-2 MONO Ab
ICDB NUMBER: 3042711
REACTIVITY : Mouse gamma1 heavy chain of immunoglobulin (determined by
immunodot), positively tested on BALB/c and C57Bl/7 mouse sera.
The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.
LO-MG1-2 and LO-MG1-13 do not bind to the same epitope.
AVIDITY: on mouse IgG1: 9 x 108 M-1 Cf. avidity sheet, for more details
CROSS-REACTIVITY: does not cross react with a normal serum of rat and rat IgG1,
IgG2a, IgG2b, IgM and IgA secreting hybridomas supernatants. It cross-reacts
with highly concentrated rat IgG2c supernatant. It does not bind to chicken,
rabbit, goat, sheep, bovine, horse, dog IgG and human IgM. It binds to swine,
human IgG and weakly baboon IgG (ELISA test).
APPLICATIONS: Cf REACTIVITY
- CAN BE LABELLED WITH FITC
- CAN BE LABELLED WITH BIOTIN
- CAN BE LABELLED WITH PEROXIDASE
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgG1 (Solid phase Sepharose 4b CNBr act.) - capacity: see data sheet.
LYOPHILIZATION: Yes, with a low possible denaturation.
REFERENCE
(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed.,29th collloquium 1981 Pergamon Press Oxford and N.Y. : 615-618
Selected references of scientific publications in which LO-MG1-2 MAb was used
Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182
Denis O. et al. Int. Immunol., 1993, 5 (1): 71-78.
El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76
Vanhove B., Bazin H. Immunology 1993, 79: 580-586.
For more information, see: Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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