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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DESIGNATION OF CELL LINE AND PRODUCT : LO-MG1-15, new cat#: 10R-I109a          $484.00/1mg

IMMUNOGEN  SUBSTANCE NAME : purified mouse IgG1 from BALB/c mice

GENUS SPECIES : Mus musculus - mouse


IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C


IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a

NAME FOR CELL LINE : LO-MG1-15 HYBRIDOMA

NAME FOR PRODUCT : LO-MG1-15 MONO Ab

ICDB NUMBER: 3003929


REACTIVITY : Mouse gamma1 heavy chain of immunoglobulin (determined by immunodot)

The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses.


CROSS-REACTIVITY: it binds to swine and human IgG. Does not bind to rabbit, bovine IgG or human IgM (ELISA test).


AVIDITY: on mouse IgG1: 2.8 x 109 M-1


APPLICATIONS : Cf REACTIVITY

- CAN BE LABELLED WITH BIOTIN

- CAN BE LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAPTURE ELISA: GOOD BINDING ON PLASTICS


LYOPHILIZATION: not tested


REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed.,29th collloquium 1981 Pergamon Press Oxford and N.Y. : 615-618


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

FOR RESEARCH  USE ONLY


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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