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RAT anti-MOUSE ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DESIGNATION OF CELL LINE AND PRODUCT : LO-ME-3, new cat#: 10R-I105a $484.00/1mg
IMMUNOGEN SUBSTANCE NAME : purified IgE (Mouse IgE) from BALB/c mice
GENUS SPECIES : Mus musculus - mouse
IMMUNOCYTE DONOR GENUS SPECIES : Rattus norvegicus- rat
STRAIN : LOU/C
IMMORTAL CELL PARTNER
DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)
HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY
CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG1, allotype IgK-1a
NAME FOR CELL LINE : LO-ME-3 HYBRIDOMA
NAME FOR PRODUCT : LO-ME-3 MONO A
ICDB NUMBER: 3003927
REACTIVITY : Mouse epsilon heavy chain of immunoglobulin (determined by
immunodot)
LO-ME-2 and LO-ME-3 do not bind to the same epitope.
LO-ME-3 doen't cross-react with rat IgE.
CROSS-REACTIVITY: does not bind to human IgG and IgM. Does not bind to chicken,
rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).
AVIDITY: on mouse IgE: 3 x 109 M-1 Cf. avidity sheet, for more details
APPLICATIONS: Cf REACTIVITY
- CAPTURE ELISA: GOOD BINDING ON PLASTICS
- CAN BE LABELLED WITH BIOTIN
- CAN BE LABELLED WITH PEROXIDASE
- CAN BE LABELLED WITH FITC
- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS
- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)
LYOPHILIZATION: yes.
REFERENCE
(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618
Selected references of scientific publications in which LO-ME-3 MAb was used
Chavez M. et al. Int. Arch. All. Immunol. 1992,97:330-336.
El Bouhdidi et al. Parasite Immunology 1994, 16: 69-76
Pierre P. et al. Eur. J. Immunol., 1992, 22: 3179-3182
Van Mechelen M. et al. Int. Immunol. 1995, 7: 199-205.
For more information, see: Rat Hybridomas and Rat Monoclonal
FOR RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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