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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-ME-2

IMMUNOGEN SUBSTANCE NAME : purified mouse IgE from BALB/c mice

GENUS SPECIES : Mus musculus - mouse


IMMUNOCYTE DONOR  GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

IMMORTAL CELL PARTNER DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgG2a, allotype IgK-1a

NAME FOR CELL LINE : LO-ME-2 HYBRIDOMA

NAME FOR PRODUCT : LO-ME-2 MONO Ab

ICDB NUMBER: 3003926


REACTIVITY : Mouse epsilon heavy chain of immunoglobulin (determined by immunodot)

LO-ME-2 and LO-ME-3 do not bind to the same epitope.


CROSS-REACTIVITY: does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).


AVIDITY: on mouse IgE: 2 x 109 M-1   Cf. avidity sheet, for more details



APPLICATIONS : Cf REACTIVITY

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE

- CAN BE LABELLED WITH BIOTIN

- CAN BE LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE USED IN IMMUNOAFFINITY CHROMATOGRAPHY for purification of mouse IgE (solid phase Sepharose 4B CNBr act.).


LYOPHILIZATION: Yes.


REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618

Selected reference of scientific publication in which LO-ME-2 MAb was used

Chavez M. et al. Int. Arch. All. Immunol. 1992,97:330-336.

Goboriau-Routhiau V. and Moreau M.C. Pediatric Research 1996,39:625.

Moreau-M.C. and Goboriau-Routhiau V. Res. Immunol. 1996,147:49.


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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