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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

DESIGNATION OF CELL LINE AND PRODUCT : LO-MD-6

IMMUNOGEN SUBSTANCE NAME: IgD (MOUSE DELTA HEAVY CHAIN OF IMMUNOGLOBULIN)

TEPC 1017, TEPC 1033 (1)

GENUS SPECIES : Mus musculus - mouse


IMMUNOCYTE DONOR

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C


IMMORTAL CELL PARTNER

DESIGNATION : Non secreting LOU/C rat IR983F fusion line (2)


HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat kappa IgG2a, allotype IgK-1a

NAME FOR CELL LINE : LO-MD-6 HYBRIDOMA

NAME FOR PRODUCT : LO-MD-6 MONO Ab

ICDB NUMBER: 3046717


REACTIVITY : Mouse delta heavy chain of immunoglobulin (determined by immunodot)


CROSS-REACTIVITY: Does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).


AVIDITY (3): on Mouse IgD : 1.2 x 109 M-1 by BIACORE (Pharmacia)*.


APPLICATIONS : Cf REACTIVITY

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH BIOTIN

- CAN BE LABELLED WITH PEROXIDASE AND FITC.

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- IMMUNOFFINITY CHROMATOGRAPHY for purification of mouse IgD (solid phase Sepharose 4b CNBr act.) - capacity: see data sheet.

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE


REFERENCES

(1) Finkelman F.O., Kessler J.W., Mushinski J.F. and Potter M. IgD-secreting murine plasmacytomas: identification and partial characterization of two IgD myeloma proteins. J. Immunol. 1981, 126: 680.

(2) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids 1982, Peeters E. ed., 29th Colloquium 1981, Pergamon Press, Oxford and N.Y., pp. 615- 618.

(3) Van Heymingen et al. J. Immunol. Methods 1983, 62: 147-153.

Selected references of scientific publications in which LO-MD-6 MAb was used

Goroff D.K., Holmes, J.M., Bazin, H., Nisol, F., Finkelman, F.D. J. Immunol., 1991, 146 : 18-25 (in this paper, LO-MD-6 is named Hbd-6); Denis O., Latinne D., Nisol F., Bazin H. Int. Immunol., 1993, 5 (1): 71-78; Latinne D. et al. Immunol. Rev. 1994, 141: 95-125.


For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.


*Kindly determened by Th. Delaunay (INRA, France).


FOR RESEARCH  USE ONLY

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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