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  RAT anti-MOUSE ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DESIGNATION OF CELL LINE AND PRODUCT : LO-MA-10, new cat#: 10R-I102a          $484.00/1mg

IMMUNOGEN

SUBSTANCE NAME : purified polyclonal IgA from BALB/c mice

GENUS SPECIES : Mus musculus - mouse


IMMUNOCYTE DONOR

GENUS SPECIES : Rattus norvegicus - rat

STRAIN : LOU/C

 

IMMORTAL CELL PARTNER

DESIGNATION : non secreting LOU/C rat IR983F fusion line (1)

 

HYBRIDOMA CELLS AND MONOCLONAL ANTIBODY

CLASS OF ANTIBODY PRODUCED : Rat Kappa IgM, allotype IgK-1a

NAME FOR CELL LINE : LO-MA-10 HYBRIDOMA

NAME FOR PRODUCT : LO-MA-10 MONO Ab

ICDB NUMBER: 3046715

 

REACTIVITY : Mouse alpha heavy chain of immunoglobulin (determined by immunodot), positively tested on BALB/c and C57Bl/7 mouse sera.

 

CROSS-REACTIVITY: does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).

 

AVIDITY: on mouse IgA: 2.9 x 109M-1

 

APPLICATIONS : Cf REACTIVITY

- CAPTURE ELISA: GOOD BINDING ON PLASTICS

- CAN BE LABELLED WITH PEROXIDASE AND BIOTIN

- CANNOT BE LABELLED WITH FITC

- CAN BE USED AS SECOND ANTIBODY IN IMMUNOASSAYS

- CAN BE COATED ON NITROCELLULOSE (DOT-ELISPOT)

- CAN BE USED ON CRYOSECTIONS (IMMUNOHISTOLOGY) AS FIRST ANTIBODY LABELLED WITH PEROXIDASE

 

LYOPHILIZATION: not tested

 

REFERENCE

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y. : 615-618

 

For more information, see: Rat Hybridomas and Rat Monoclonal Antibodies. Bazin H. (Ed.), CRC Press, Boca Raton, Florida, USA, 1990, 515 pages.

 

FOR RESEARCH  USE ONLY


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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