rev:   July 8, 2003

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INSULIN  RELATED ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


MONOCLONAL ANTI-Human Proinsulin

catalog# RDI-TRK2P9-3A1      clone 3A1

               RDI-TRK2P9-1G4     clone 1G4

               RDI-TRK2P9-1D6     clone 1D6

               RDI-TRK2P9-1D3     clone 1D3

$375.00/1mg   $344.00/mg 2-4

Custom Biotin labeling on request $625.00/1mg minimum 3mgpackaging: 1mg of purified antibody in 0.2ml (5mg/ml)

PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.

Purification: Protein A sepharose

Clones(isotype) 3A1, 1G4, 1D6 or 1D3 (all mIgG1 kappa)

derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of a Balb/c mice immunized with purified human proinsulin.

Specificity: -Both clones are directed to different antigenic determinants.

-Clone 3A1 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.

Clones 1D6 and 1D3 Mab react with different epitopes on human proinsulin molecule, both of each are generated by the interactions between insulin and C-peptide parts of pro-hormone.

-Kd for clone 3A1 is 1.1 X 10 E-08M

-Kd for clone 1G4 is 2.6 X 10 E-07M

-Kd for clone 1D6 and 1G4 >3 X 10 E-08M

-These clones do not cross react with human, bovine, porcine insulin or bovine and porcine proinsulin or human c-peptide.

Application: Detection of human proinsulin in two site enzyme immunoassay.

Assay recommendations:

- MoAb 3A1 for coating and anti-insulin MoAb 7F8 (Ca#RDI-TRK2IL-7F8) for labeling.

- MoAb 1G4 for coating and mixture of antibodies anti-insulin/proinsulin MoAb D6C4 and D3E7

(Cat#RDI-TRK2IP10-D6C4 or RDI-TRK2IP10-D3E7 - $375.00/mg) for labeling.

- 1D6 or 1D3 Mabs are used for direct capture prohormone from serum sample and insulin-specific 7F8, D6C4 or D3E7 Mabs (Cat# RDI-TRK2I1-7F8, RDI-TRK2IP10-D6C4 or -D3E7) as a conjugates.

Antibodies 3A1 and 1G4 can be used in immunohistochemistry on frozen sections.

Antibodies 1D6 and 1D3 can be also used for:

- Construction of a sensitive two-site sandwich enzyme-linked/RIA immunosorbent assays for the measurement of intact human proinsulin in serum or plasma with the detection limit in buffer 0.4 - 0.5 pmol/L.

- Specific immunosorbtional (immobilized 1D6/1D3 Mabs) removal of proinsulin molecules from serum samples before the measurement of insulin C-peptide.

Clones 3A1 and 1G4 can be used in immunohistochemistry on frozen sections.

Safety: Although this antibody contains low amounts of sodium azide (0.1%), appropriate care must be taken when using this product. Avoid use of metal drains and flush with copious amounts of water.

Note:Upon receipt, tighten cap and centrifuge for 1-2 minutes at 1000 rpm to concentrate antibody in bottom of vial. If desired, can add PBS with 0.1% sodium azide to bring concentration to 1mg/ml.


All material is for in vitro research use only-not for use in or on humans or animals-not for diagnostics. Not responsible for any patent infringements for the use or derivation of these products.


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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