rev: May 14, 2001

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INSULIN  RELATED ANTIBODIES  

(anti-Human and others as indicated)

Research Diagnostics Inc  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Rabbit anti-Insulin Receptor Beta chain

cat#RDI-INSRBabr       $325.00/200ug vial -rabbit IgG

-specific for the insulin receptor Beta chain, non cross reactive with the insulin receptor alpha chain.

-recognizes epitope corresponding to amino acids 1365-1382 at the carboxy terminus of insulin receptor beta chain of human origin (differs from the corresponsing mouse sequence by a single amino acid).

-reactive with human, rat and mouse

-suitable for western blotting and immunohistochemistry

-control peptide for blotting available cat#RDI-INSRB/CP $100.00/100ug

also available agarose conjugated cat#RDI-INSRB-AGR $375.00/500ug in 0.25ml


Rabbit anti-Insulin Receptor BETA Chain:

Background: The IGF-1 receptor is responsible for the growth effects of both IGF-1 and IGF-II and is structurally and functionally similar to the insulin receptor. Both receptors are 350 kDa glycoproteins composed of two extracellular alpha subunits and two transmembrane Beta subunits. The cytoplasmic protein tyrosine kinases are activated by ligand binding to the extracellular domains of the receptors. Kinase activation in turn stimulates an intracellular cascade of molecular interactions involving multiple effects of IGFs and insulin. Insulin effects metabolic effects such as increases in glucose uptake and glycogen synthesis, whereas IGFs are more potent stimulators of mitogenic activity. However in cells that express both insulin and IGF receptors, a certain degree of overlap in these biological activities is observed.

Cat#: #RDI-INSRBabr     $325.00/200ug in 1ml vial


Derivation: Produced in rabbit using a synthetic peptide corresponding to amino acids 365-1382 mapping at the carboxy terminus of the insulin receptor beta chain of human origin (differs from the corresponding mouse sequence by a single amino acid) Affinity purified.

Presentation: Each vial contains 200ug IgG in 1.0ml 0.05M PBS with 0.2% gelatin and 0.1% sodium azide.

Specificity: Specifically recognizes the insulin receptor beta chain of mouse, rat and human origin by western blot and immunohistochemistry, non reactive with the Insulin receptor alpha chain.

Storage: STORE at 4 DEG C-DO NOT FREEZE. Stable for one year from date of shipment.

General REF:
Lammers, R. et al, EMBO J. 8:1369-1375.

Ullrich, A. et al, EMBO J. 5:2503-2512


FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-Not FOR DIAGNOSTIC PROCEDURES.


also available: control peptide for competition cat#RDI-INSRB-CP $100.00/100ug in 0.5ml PBS with 100ug BSA and 0.1% sodium azide). $75.00 with antibody

-use by first titering antibody to minimal amount needed, then use a 10X weight excess of peptide antigen in PBS, preincubate 2 hours at room tempor overnight at 4 DEG C with antibody. Then proceed with test.


Research Diagnostics Inc

Pleasant Hill Road

Flanders NJ 07836

phone (800) 631-9384 or (973) 584-7093

fax     (973) 584-0210

EMAIL:ResearchD@aol.com

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