rev: May 14, 2008
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INSULIN RELATED ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
MONOCLONAL ANTI-Human insulin
catalog# RDI-TRK2I1-C7C9, new cat#: 10R-I134f
clone C7C9 $412.50/1mg
RDI-TRK2I1-E6E5, new cat#: 10R-I134e clone E6E5 $412.50/1mg
RDI-TRK2I1-D4B8, new cat#: 10R-I134a clone D4B8 $412.50/1mg
RDI-TRK2I1-7F5 clone 7F5
RDI-TRK2I1-3A6, new cat#: 10R-I134d clone 3A6 $412.50/1mg
RDI-TRK2I1-8E2, new cat#:
10R-I134b clone 8E2 $412.50/1mg
packaging: 1mg of purified antibody at 1 mg/ml in 1.0ml PBS, pH 7.4, containing
0.1% of sodium azide as preservative. Store at 4 DEG
C.
Purification: Protein A sepharose
Clones(isotype): all mIgG1
kappa
Specificity: Clone InsC7C9 reacts with c-terminal pentapeptide
of insulin B-chain with Kd=10-9M. Clone C7C9 also
cross reacts with bovine and porcine insulin.
Clone D4B8 Kass= 1.5 X 10-9M detecting
Clone E6E5 Kd= 7.5 X 10-9M capture/coating
Clone 7F5, kass=1 X 10 E0 9M
clone 3A6 Kass=8 X 10E 08M capture ab
Clone 8E2 Kass=6 X 10E 08M conjugate
These antibodies are directed to different antigenic determinants. These antibodies cross react human proinsulin, bovine insulkin (30%) and porcine insulinApplication: -Detection of insulin in two site enzyme immunoassay. We recommend using clone E6E5 for coating and D4B8 for labeling. or clones 3A6 (coating) and clone 8E2 (detecting)
Clone D4B8 and E6E5 can be used in histochemistry on frozen sections.
Safety: Although this antibody contains low amounts of sodium azide (0.1%), appropriate care must be taken when using this product. Avoid use of metal drains and flush with copious amounts of water. All material is for in vitro research use only-not for use in or on humans or animals-not for diagnostics. Not responsible for any patent infringements for the use or derivation of these products. supply contract or standing orders are also encouraged.
Mouse anti-INSULIN (Monoclonal Cocktail)
cat# RDI-INSCOCabm $344.00/100ug
RDI-INSCOCabm-1 $500.00/200ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Isotype: mIgG1 (both)
Clone: E2-E3 & 2D11-H5
Reactivity: human and pig (does not react with mouse and rat) (others not tested)
Uses: -histochemistry (frozen and paraffin sections) approx 1:50-1:100 60 minutes RT
Positive Ctrl: pancreas
Localization: Cytoplasmic
Background: Recognizes a polypepide identified as insulin. Insulin is a 51 amino acid polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic redidue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. The main storage site for insulin is the pancreatic islets.
Storage: 2-8 deg c
For Research Use Only
-also available: monoclonal anti-insulin and IGFI, IGF2, ILGFR abs
-see also new monoclonals against insulin (human, chicken, porcine and rat) suitable for paraffin sections cat#RDI-INSLE2abm (clone E2-E3) $438/100ug $688.00/200ug or cat#RDI-INSL2Dabm (clone 2D11-H5) $438.00/100ug $688.00/200ug NOTE:Both clones see different epitope on insulin.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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