rev: 5/16/2008
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INSULIN RELATED ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
DATA SHEET: Mouse anti-Rat/Human Insulin Like Growth Factor II
Catalog#: RDI-ILGF2abm
Price: $594.00/vial
Package Size: 500 micrograms (in sterile filtered PBS buffer, no preservatives
or carrier added)
Species: mouse IgG1 purified via ammonium sulphate precipitation and
DEAE-cellulose chromatography.
Antigen: partially purified rat IGF-II
Specificity: specific for IGF-II of rat and human origin (kd=5.9
X 10E9); less than 10% cross with human IGF-I.
Activity: Neutralization: 100 micrograms/ml to inhibit the stimulatory effect
of 100 nanograms IGF-II/ml on DNA synthesis of human fibroblasts and sulfation
by chick cartilage.
Western blot: 2 micrograms/ml to detect 10- 100ng IGF-II Immunocytochemistry:
0.1ug/ml
-MUST TITER FOR EACH APPLICATION
Storage: Upon receipt, store at -20 DEG C or below. -After reconstitution,
aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
MOUSE MONOCLONAL ANTIBODY TO:
HUMAN INSULIN-LIKE GROWTH FACTOR II
PRODUCT CODE RDI-CBL82 $525.00/vial
CLONE W2H1
ISOTYPE IgG1
IMMUNIZING ANTIGEN Human anti-globulin conjugated to IGF-II
FUSION PARTNER NS1 myeloma cell line
SOURCE Mouse ascitic fluid
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY
The antibody recognises IGF-II, cross-reactivity with IGF-I <0.01% and insulin <0.01%.
APPLICATIONS
*Measurement of serum IGF-II levels
*Suitable for use in RIA, ELISA, IRMA or immuno-histochemistry (frozen sections only)
PRESENTATION The monoclonal is presented at a concentration of 0.1mg in 0.5ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE
For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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