rev:   January 13, 2004

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INSULIN  RELATED ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DATA SHEET: Mouse anti-Rat/Human Insulin Like Growth Factor II


Catalog#: RDI-ILGF2abm

Price: $594.00/vial  

LOT# see batch sheet

Package Size: 500 micrograms (in sterile filtered PBS buffer, no preservatives or carrier added)

Species: mouse IgG1 purified via ammonium sulphate precipitation and DEAE-cellulose chromatography.

Antigen: partially purified rat IGF-II

Specificity: specific for IGF-II of rat and human origin (kd=5.9 X 10E9); less than 10% cross with human IGF-I.

Activity: Neutralization: 100 micrograms/ml to inhibit the stimulatory effect of 100 nanograms IGF-II/ml on DNA synthesis of human fibroblasts and sulfation by chick cartilage.
Western blot: 2 micrograms/ml to detect 10- 100ng IGF-II Immunocytochemistry: 0.1ug/ml

-MUST TITER FOR EACH APPLICATION

Storage: Upon receipt, store at -20 DEG C or below. -After reconstitution, aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


MOUSE MONOCLONAL ANTIBODY TO:

HUMAN INSULIN-LIKE GROWTH FACTOR II

PRODUCT CODE    RDI-CBL82    $375.00/vial

CLONE      W2H1

ISOTYPE   IgG1

IMMUNIZING ANTIGEN  Human anti-globulin conjugated to IGF-II

FUSION PARTNER   NS1 myeloma cell line

SOURCE  Mouse ascitic fluid

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY

The antibody recognises IGF-II, cross-reactivity with IGF-I <0.01% and insulin <0.01%.

APPLICATIONS

*Measurement of serum IGF-II levels

*Suitable for use in RIA, ELISA, IRMA or immuno-histochemistry (frozen sections only)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 0.1mg in 0.5ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE

For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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