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INSULIN  RELATED ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

& SGLT-1 & SGLT-2 (see other page for glucose 4 antibodies)

Catalog Number: RDI-ALS24372      $312.00/100ug  

                            RDI-ALS24372X   $1875.00/1mg

Lot Number:

Quantity: 100 mg in 100 ml (1 mg/ml) in PBS containing 0.02% sodium azide.

Description: Non-insulin-dependent diabetes mellitus (NIDDM) is a multifactoral disease with both environmental and genetics causes. Genome-wide screening procedures have identified several susceptibility loci for NIDDM within the human genome. A putative sugar transporter that has been localized to human chromosome 20q12-q13.1, one of the genomic loci associated with NIDDM. Because of the strong resemblance of this novel protein to members of the mammalian facilitative glucose transporter family (GLUT), the protein is known as GLUT10 (HGMW-approved gene symbol SLC2A10). Data suggests that GLUT10 an excellent candidate for a susceptibility gene involved in NIDDM.

Host Species: Rabbit

Specificity: Residues with residues 367-385 (ILSTAKKTKPHPRSGDPSA) of the human GLUT10 protein.

Applications: Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

References: (1) McVie-Wylie,A.J., Lamson,D.R. and Chen,Y.T. (2001). Genomics 72, 113-117.

                    (2) Wood,I.S., Hunter,L. and Trayhurn,P. (2003) Biochem. Biophys. Res. Commun. 308 (1), 43-49.

For research use only; not for use as a diagnostic.

Package Size: 20ug IgG aff pure material in ).15M PBS ph 7.2, w/ 0.2% soidum azide and 0.05% BSA.

Lot: see sheet with shipment

Activity: Reacts only with human Glut 3, does not cross react with Glut 3 from rat or mouse. Exhibits only a single band of Mr 47Kda on western blot. Useful for study of Glut 3 in human brain and specific human tumor cell lines.

Use: -Western blot: approx 1:5K dilution against whole human brain homogenates.

-Dot blot: 1:5K against peptide-BSA.

-immunocytochemistry:1:500 against human intestinal tumor cells.

Antigen: A 12 amino acid synthetic peptide corresponding to the carboxy terminus of the human GLUT 3 sequence ( SIEPAKETTNV) conjugated to keyhole limpet hemocyanin via a cysteine on the amino terminus of the peptide.

Storage: -20 DEG C long term. 4 DEG C short term (recommend adding a preservative ). AVOID FREQUENT FREEZE THAW CYCLES.

ref:

-Kayano, T. et al, J. Biol. Chem. 263:15245- 15248, 1988.

-Harris, D.S. et al, Proc. Natl. Acad. Sci. 89:7556-7560, 1992

-Mantych, G.J. et al, Endocrinology 131:1270- 1278, 1992.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Catalog#: RDI-GLUT3MabR    -sold out-

-immunoprecipitation: used for in vitro translation

-immunohistochemistry: 1:500 against rat cultured pancreas and liver cells.

Antigen: A fusion protein consisting of the carboxy terminal 25 amino acids of the rat Glut 2 glucose transporter and glutathione S transferase. Some subsequent boosts were with the synthetic peptide CVQMEFLGSSETV.

Storage: -20 DEG C long term. 4 DEG C short term (recommend adding a preservative ). AVOID FREQUENT FREEZE THAW CYCLES.

ref: -Thorens, B. et al, Cell:55, 281-290, 1988.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Package Size: 100ul neat serum

Lot: see sheet with shipment

Activity: Stains Glut 5 as found in homogenates from   Caco-2 human intestinal cell lines. Does not cross react with Glut 1, 2, 3 or 4 from human, mouse or rat.

Use: -Western blot: approx 1:500-1:1.5K against   human jejunum and kidney; no reaction against brain, gut or muscle from rat. Does not react with human blood, fat, liver or muscle. Also reacts with testis, human intestinal cell homogenates and spermatozoa homogenates.
        -histochemistry: 1:100 against paraffin embedded jejunum sections.

Antigen: A 12 amino acid synthetic peptide corresponding to the carboxy terminus of the human GLUT 5 with a cysteine included at the N-terminus for conjugation.

Storage: -20 DEG C long term. 4 DEG C short term (recommend adding a preservative ). AVOID FREQUENT FREEZE THAW CYCLES.

ref:

-Kayano, T. et al, J. Biol. Chem. 265:13276- 13282, 1990

-Burant, C. F. et al, J. Biol. Chem, July/Aug 1992

-Harris, D.S. et al, Proc. Natl. Acad. Sci. 89:7556-7560, 1992.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6/SLC2A6, originally described as glut-9 has been reassigned as Glut-6. Glut-6 (human 507 aa; ~ 48 kDa; chromosome 9q34) is most closely related to Glut-8 (~45% homology). It is highly expressed in brain, spleen, and peripheral leukocytes. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leucocytes and brain. Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496 aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617 aa, , monkey 621 aa;~ 50 kDa; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine . Glut-13 or proton myo-inositol transporter (HMIT; human 629 aa, rat 618 aa, ~75-90 kDa/67 kDa protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol.

For A review of the nomenclature and details see Joost, H-G et al (2002) Am. J. Endo. Metabol. 282, E974-E976

References:

Glut-1: Mueckler M et al (1985) Science 229, 941-985; Fukumoto,H et al (1989) Diabetes 37, 657-661 Glut-2: Fukumoto H et al (1989) JBC 264, 7776-7779

Glut-3: Kayano T et al (1988) JBC 263, 15245-15248

Glut-4: Fukumoto H et al (1989) JBC 264, 7776-7779; Buse JB et al (1992) Diabetes 41, 1436-1445; Chiaramonte,R. et al (1993) Gene 130, 307-308; Choi WH et al (1991) Diabetes 40, 1712

Glut-5: Kayano T et al (1990) JBC 265, 13276-13282

Glut-6: Doege H et al (2000) Biochem. J. 350, 771-776

Glut-7: Waddell ID et al (1992) Biochem. J. 286, 173-177

Glut-8: Carayannopoulos MO et al (2000) PNAS 13, 7313-7318; Doege H et al (2000) JBC 275, 16275-16280; Ibberson M et al (2000) JBC 275, 4607-4612

Glut-9: Phay JE et al (2000) Genomics 66, 217-220

Glut-10: McVie-Wylie AJ et al (2001) Genomics 72, 113-117

Glut-11: Doege H et al (2001) Biochem J. 359, 443-459

Glut-12: Rogers S et al (2002) Am. J. Endocrinol. Metabol. 282, E733-E738

Glut-13: Uldry M et al (2001) EMBO J. 20, 4467-4477

Rabbit anti-Mouse Glut-1    cat#RDI-MGLUT1Cabr   $469.00/vial 100ug IgG

Antigen/regions: M (CT,12 aa);

Antibody Cross reactivity: M, R, H, Rb, B, P, C

Control Peptide (100 µg): Catalog No. RDI-MGLUT1C-CP $156.00

Antigen/regions: H (CT, 15 aa);

Antibody Cross reactivity: M, R, H, Rb, B, P

Control Peptide (100 µg): Catalog No. RDI-GLUT1C-CP $156.00

Antigen/regions: R (I, 16 aa);

Control Peptide (100 µg): #RDI-RTGLUT2-CP $156.00

Antigen/regions: H (CT, 14 aa);

Antibody Cross reactivity: H (R, M, Weak)

Control Peptide (100 µg): #RDI-GLUT2-CP $156.00

Antigen/regions: M (CT, 12 aa);

Antibody Cross reactivity: M, R

Control Peptide (100 µg):#RDI-MGLUT3-CP $156.00

Rabbit anit-mouse GLUt-3   cat#RDI-MGLUT3abrXP   $469.00/100ug aff pure

Antigen/regions: H (CT, 12 aa);

Antibody Cross reactivity: H Neat Serum (100 µl): Catalog Nr. GT32-S;

Control Peptide (100 µg): #RDI-GLUT3-CP $156.00

Antigen/regions: M (CT, 12 aa);

Antibody Cross reactivity: M, R, H

Control Peptide (100 µg): #RDI-MGLUT4-CP $156.00

Antigen/regions: R (CT, 13 aa);

Antibody Cross reactivity: M, R

Control Peptide (100 µg):#RDI-RTGLUT5-CP $156.00

Antigen/regions: H (CT, 12 aa);

Antibody Cross reactivity: H

Control Peptide (100 µg):#RDI-GLUT5-CP $156.00

Antigen/regions: H (CT, 16 aa);

Antibody Cross reactivity: H

Control Peptide (100 µg):#RDI-GLUT6-CP $156.00

Antigen/regions: R (CT, 14 aa) sequence is identical in human

Antibody Cross reactivity: R (H?)

Control Peptide (100 µg): #RDI-RTGLUT7-CP $156.00

Antigen/regions: R (CT, 12 aa) sequence is identical in human

Antibody Cross reactivity: R (H?)

Control Peptide (100 µg):#RDI-RTGLUT7B-CP: $156.00

Antigen/regions: M (CT, 11 aa); peptide sequence is 90% conserved in rat and human Glut-8

Antibody Cross reactivity: M, rat human

Control Peptide (100 µg):#RDI-MGLUT8-CP $156.00

Antigen/regions: H (CT, 21 aa);

Antibody Cross reactivity: Human

Control Peptide (100 µg):#RDI-GLUT9-CP $156.00

Antigen/regions: H (CT, 16 aa);

Antibody Cross reactivity: Human

Control Peptide (100 µg):#RDI-GLUT10-CP $156.00

Antigen/regions: Human(CT, 16 aa);

Antibody Cross reactivity: Human

Control Peptide (100 µg):#RDI-GLUT11-CP $156.00

Antigen/regions: Human(CT, 18 aa); peptide sequence is 100% conserved in monkey Glut-12, 71% mouse, 69% rat

Antibody Cross reactivity: human

Control Peptide (100 µg):#RDI-GLUT12-CP $156.00

Antigen/regions: Human (CT, 16 aa); peptide sequence is 100% conserved in rat Glut-13/HMIT

Antibody Cross reactivity: Human

Control Peptide (100 µg):#RDI-GLUT13-CP $156.00

Antigen/regions: Rb (I, 19 aa)

Antibody Cross reactivity: M, R, H Rb, D

Control Peptide (100 µg):#RDI-SGLT1-CP $156.00

Rabbit anti-PIG SGLT-2   cat#RDi-SGLT2abrX $469.00/100ug

M=Mouse;  R=Rat;   H=Human,   Rb=Rabbit,  D=Dog;   P=Pig;  C=Chicken;

CT=C-teminal;   NT=N-terminal;   I=Internal;

All products are for In vitro research use only. Not for human or drug use.

HUMAN INSULIN REGULATABLE GLUCOSE TRANSPORTER 1 (GLUT-1)

PRODUCT CODE                RDI-CBL242     $375.00

HOST                                  Rabbit

IMMUNIZING ANTIGEN   12 amino acid peptide of the C-terminus of GLUT-1 conjugated to KLH

PURIFICATION METHOD Affinity purified on a Sepharose-4B CNBr linked peptide column

SPECIFICITY The affinity purified antisera is specific for human glucose transporter 1 from muscle and adipose tissue. Does not cross-react with GLUT-4.

APPLICATIONS *Western blot analysis of GLUT-1 activity in adipose and muscle tissue (5ug/ml)*Immunoprecipitation of GLUT-1 (5ug/ml)

KNOWN SPECIESCROSS REACTIVITY The antisera is known to crossreact with rat and mouse GLUT-1

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 100µg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

RDI Div of FItzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

phone (978) 371-6446

fax     (978) 371-2266

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