rev: August 11, 1999

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INSULIN  RELATED ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

PRODUCT CODE :RDI-CBL71    $375.00/vial

CLONE: MAB 1 was produced by immunization of BALB/c mice with biosynthetic human insulin, spleen and lymph node cells were fused with myeloma cell line 63 Ag 8.6.5.3.

ISOTYPE: IgG1

SOURCE: Tissue culture supernatant

AFFINITY CONSTANT: Human Insulin 1.8 x 109

                                          Porcine Insulin <1 x 106

                                          Bovine Insulin <1 x 106

                                          Human Proinsulin <1 x 101

PURIFICATION METHOD: Protein A affinity chromatography

SPECIFICITY: MoAb 1 recognizes an epitope including the amino acid B30. If B30 is lacking (Des-B30 insulin), cross-reaction is diminished to 7.5%. If B30 is linked to the C-peptide (proinsulin), the epitope can no longer be recognized (cross-reaction of 1.1%). The addition of two amino acids in positions B31 and B32 (di-Arg insulin) alters the epitope (cross-reaction diminished to 0.4%). The exchange of Thr-B30 to a simple amino acid without sidechain leads to reduced binding (Gly, 5.4%). This fact indicates that the binding site is not formed by the carboxyl group of the amino acid. Correct epitope structure depends on a side chain of B30. Regarding MoAb 1, the essential part of the side chain is the hydroxyl group of Thr-B30: a loss of the methyl group going from Thr (100%) to Ser (22%) still allows binding. An additional loss of the hydroxyl group going to Ala (3.2%) destroys the epitope.

The difference between Ser and Thr recognition may be due to the differing steric position of the hydroxyl group. The importance of the hydroxyl group can also be seen by the weak binding of MoAb 1 to Val-B30 insulin (8.5%), where the hydroxyl group is replaced by a methyl group.

APPLICATIONS: RIA and EIA assay development.

PRESENTATION: 1 mg of murine monoclonal antibody (A280nm) dissolved in phosphate buffered saline. Contains 10mM sodium azide as preservative.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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